Ser221
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Home > Phosphorylation Site Page: > Ser221  -  p90RSK (rat)

Site Information
DHEKKAYsFCGTVEY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447988

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , mutation of modification site ( 1 , 2 , 3 ) , phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
p90RSK (human) ( 3 ) , PDK1 (human) ( 1 )
Treatments:
BX795 ( 1 ) , EGF ( 1 ) , PD98059 ( 3 ) , phorbol_ester ( 3 )

Downstream Regulation
Effects of modification on p90RSK:
enzymatic activity, inhibited ( 2 ) , intracellular localization ( 1 ) , molecular association, regulation ( 1 , 2 )
Effects of modification on biological processes:
apoptosis, inhibited ( 2 )
Induce interaction with:
PKAR1A (cow) ( 2 )
Inhibit interaction with:
PKAR1A (human) ( 1 )

References 

1

Gao X, Chaturvedi D, Patel TB (2012) Localization and retention of p90 ribosomal S6 kinase 1 in the nucleus: implications for its function. Mol Biol Cell 23, 503-15
22130794   Curated Info

2

Gao X, Chaturvedi D, Patel TB (2010) p90 Ribosomal S6 kinase 1 (RSK1) and the catalytic subunit of protein kinase A (PKA) compete for binding the pseudosubstrate region of PKAR1alpha: role in the regulation of PKA and RSK1 activities. J Biol Chem 285, 6970-9
20048145   Curated Info

3

Dalby KN, et al. (1998) Identification of regulatory phosphorylation sites in mitogen-activated protein kinase (MAPK)-activated protein kinase-1a/p90rsk that are inducible by MAPK. J Biol Chem 273, 1496-505
9430688   Curated Info