Lys221
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Home > Acetylation Site Page: > Lys221  -  NFkB-p65 (human)

Site Information
LLCDkVQkEDIEVYF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 459094

In vivo Characterization
Methods used to characterize site in vivo:
3H acetate labeling ( 8 ) , immunoassay ( 4 ) , immunoprecipitation ( 4 , 8 ) , modification-specific antibody ( 4 ) , mutation of modification site ( 1 , 4 , 8 ) , western blotting ( 4 , 8 )
Disease tissue studied:
glioma ( 1 ) , melanoma skin cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
STAT3 (human) ( 4 )
Treatments:
IL-10 ( 4 ) , TNF ( 4 ) , trichostatin_A ( 4 )

Downstream Regulation
Effects of modification on NFkB-p65:
intracellular localization ( 1 , 4 , 8 ) , molecular association, regulation ( 8 )
Effects of modification on biological processes:
carcinogenesis, induced ( 4 ) , transcription, induced ( 1 , 4 , 8 )
Induce interaction with:
DNA ( 8 )
Inhibit interaction with:
IkB-alpha (human) ( 8 )

Disease / Diagnostics Relevance
Relevant diseases:
lung cancer ( 4 )

References 

1

Wollebo HS, et al. (2016) The Brd4 acetyllysine-binding protein is involved in activation of polyomavirus JC. J Neurovirol 22, 615-625
27007123   Curated Info

2

Bai HB, Wang Y, Zhang YH, Zhang Y (2016) [AMP-activated protein kinase activation regulates adhesion of monocytes to vascular endothelial cells and the underlying mechanism]. Sheng Li Xue Bao 68, 41-9
26915321   Curated Info

3

Zhang Y, et al. (2011) AMP-activated protein kinase suppresses endothelial cell inflammation through phosphorylation of transcriptional coactivator p300. Arterioscler Thromb Vasc Biol 31, 2897-908
21940946   Curated Info

4

Lee H, et al. (2009) Persistently activated Stat3 maintains constitutive NF-kappaB activity in tumors. Cancer Cell 15, 283-93
19345327   Curated Info

5

Dai Y, et al. (2008) Interactions between Bortezomib and Romidepsin and Belinostat in Chronic Lymphocytic Leukemia Cells. Clin Cancer Res 14, 549-58
18223231   Curated Info

6

Singh M, Popowicz GM, Krajewski M, Holak TA (2007) Structural ramification for acetyl-lysine recognition by the bromodomain of human BRG1 protein, a central ATPase of the SWI/SNF remodeling complex. Chembiochem 8, 1308-16
17582821   Curated Info

7

Ishinaga H, et al. (2007) TGF-beta induces p65 acetylation to enhance bacteria-induced NF-kappaB activation. EMBO J 26, 1150-62
17268554   Curated Info

8

Chen LF, Mu Y, Greene WC (2002) Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-kappaB. EMBO J 21, 6539-48
12456660   Curated Info