Lys7
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Home > Acetylation Site Page: > Lys7  -  APE1 (human)

Site Information
_MPkRGkkGAVAEDG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 459076

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 6 ) , 3H acetate labeling ( 6 ) , immunoprecipitation ( 6 ) , mass spectrometry ( 4 ) , mass spectrometry (in vitro) ( 6 ) , modification-specific antibody ( 3 , 5 ) , mutation of modification site ( 1 , 2 , 5 , 6 ) , western blotting ( 3 , 5 , 6 )
Disease tissue studied:
colorectal cancer ( 1 , 2 , 6 ) , colorectal carcinoma ( 1 , 2 , 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Treatments:
Ca(2+) ( 6 ) , H2O2 ( 5 ) , MMS ( 3 , 5 ) , nicotinamide ( 5 ) , resveratrol ( 5 ) , siRNA ( 3 , 5 , 6 ) , trichostatin_A ( 5 , 6 )

Downstream Regulation
Effects of modification on APE1:
enzymatic activity, induced ( 2 ) , enzymatic activity, inhibited ( 5 ) , molecular association, regulation ( 1 , 5 , 6 )
Effects of modification on biological processes:
cell growth, induced ( 1 , 2 ) , DNA repair, induced ( 1 , 2 ) , DNA repair, inhibited ( 5 ) , transcription, inhibited ( 6 )
Induce interaction with:
DNA ( 6 ) , XRCC1 (human) ( 1 )
Inhibit interaction with:
XRCC1 (human) ( 5 )

References 

1

Roychoudhury S, et al. (2017) Human Apurinic/Apyrimidinic Endonuclease (APE1) Is Acetylated at DNA Damage Sites in Chromatin, and Acetylation Modulates Its DNA Repair Activity. Mol Cell Biol 37
27994014   Curated Info

2

Sengupta S, et al. (2016) Elevated level of acetylation of APE1 in tumor cells modulates DNA damage repair. Oncotarget 7, 75197-75209
27655688   Curated Info

3

Lirussi L, et al. (2012) Nucleolar accumulation of APE1 depends on charged lysine residues that undergo acetylation upon genotoxic stress and modulate its BER activity in cells. Mol Biol Cell 23, 4079-96
22918947   Curated Info

4

Fantini D, et al. (2010) Critical lysine residues within the overlooked N-terminal domain of human APE1 regulate its biological functions. Nucleic Acids Res 38, 8239-56
20699270   Curated Info

5

Yamamori T, et al. (2010) SIRT1 deacetylates APE1 and regulates cellular base excision repair. Nucleic Acids Res 38, 832-45
19934257   Curated Info

6

Bhakat KK, et al. (2003) Role of acetylated human AP-endonuclease (APE1/Ref-1) in regulation of the parathyroid hormone gene. EMBO J 22, 6299-309
14633989   Curated Info