Ser211
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Home > Phosphorylation Site Page: > Ser211  -  GR (human)

Site Information
PGkEtNEsPWRSDLL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447968

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 10 ) , immunoprecipitation ( 10 , 17 ) , mass spectrometry ( 7 ) , mass spectrometry (in vitro) ( 12 ) , mutation of modification site ( 10 , 11 , 16 , 17 , 20 , 21 ) , phospho-antibody ( 2 , 4 , 5 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 17 , 18 , 19 , 20 , 21 ) , phosphoamino acid analysis ( 12 ) , western blotting ( 2 , 4 , 5 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 18 , 19 , 21 )
Disease tissue studied:
bone cancer ( 9 , 15 , 21 ) , leukemia ( 18 ) , T cell leukemia ( 18 ) , liver cancer ( 9 ) , lung cancer ( 9 , 13 , 21 ) , non-small cell lung cancer ( 9 ) , non-small cell lung adenocarcinoma ( 9 ) , lymphoma ( 18 , 20 ) , anaplastic large cell lymphoma ( 18 , 20 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 8 ) , 293 (epithelial) ( 10 ) , A549 (pulmonary) ( 9 , 13 , 21 ) , CCRF-CEM (T lymphocyte) ( 18 , 20 ) , CEM-C1-15 ( 5 , 13 ) , CEM-C7-14 ( 5 , 13 ) , COS (fibroblast) ( 11 , 19 ) , CV1 (fibroblast) ( 12 ) , fibroblast ( 2 ) , H9c2 (myoblast) ( 9 ) , HCT116 (intestinal) ( 16 ) , HeLa (cervical) ( 6 , 7 , 9 , 10 ) , HTC (hepatic) ( 9 ) , LbetaT2 ( 14 ) , liver ( 21 ) , lymph node ( 21 ) , muscle ( 4 ) , prostate ( 21 ) , S49 (T lymphocyte) ( 20 ) , thyroid ( 21 ) , U2OS (bone cell) [GR (human)] ( 17 , 21 ) , U2OS (bone cell) ( 9 , 15 )

Upstream Regulation
Regulatory protein:
PPP5C (human) ( 8 )
Putative in vivo kinases:
CDK5 (human) ( 16 ) , P38A (human) ( 12 )
Kinases, in vitro:
CDK5 (human) ( 16 ) , P38A (human) ( 12 , 20 )
Putative upstream phosphatases:
PPP5C (human) ( 17 )
Treatments:
amanitin ( 11 ) , aphidicolin ( 10 ) , calyculin_A ( 17 ) , colforsin ( 18 ) , deacylcortivasol ( 20 ) , dexamethasone ( 2 , 5 , 9 , 10 , 11 , 13 , 14 , 15 , 17 , 18 , 19 , 20 , 21 ) , etoposide ( 5 ) , fluocinolone ( 21 ) , fluticasone ( 8 ) , geldanamycin ( 17 ) , GnRH ( 14 ) , GSK-3_inhibitor_X ( 15 ) , hydrocortisone ( 19 ) , hydroxyurea ( 10 ) , JNK_inhibitor_VIII ( 10 ) , Methylprednisolone ( 2 ) , MG132 ( 11 ) , mifepristone ( 2 , 21 ) , MPA ( 19 ) , NET-A ( 19 ) , nocodazole ( 10 ) , okadaic_acid ( 17 ) , PD98059 ( 10 ) , prednisolone ( 21 ) , progesterone ( 19 ) , rapamycin ( 18 ) , SB202190 ( 10 ) , SB203580 ( 8 , 9 , 20 ) , seliciclib ( 10 ) , siRNA ( 8 ) , SP600125 ( 13 , 18 , 20 ) , taxol ( 10 ) , triamcinolone ( 2 ) , U0126 ( 18 , 20 ) , UV ( 13 ) , UVC ( 9 ) , VX-680 ( 6 ) , ZK299 ( 21 )

Downstream Regulation
Effects of modification on GR:
activity, induced ( 10 ) , activity, inhibited ( 16 ) , intracellular localization ( 5 , 6 ) , molecular association, regulation ( 11 ) , protein conformation ( 12 ) , protein stabilization ( 12 )
Effects of modification on biological processes:
apoptosis, induced ( 20 ) , cell cycle regulation ( 10 ) , transcription, altered ( 12 ) , transcription, induced ( 2 , 8 , 10 , 11 ) , transcription, inhibited ( 16 )
Induce interaction with:
GRIP1 (human) ( 11 )

References 

1

Nicoll JX, Fry AC, Mosier EM (2021) Androgen and glucocorticoid receptor phosphorylation following resistance exercise and pre-workout supplementation. Steroids 172, 108859
33974920   Curated Info

2

Nakamura R, et al. (2020) Complex fibroblast response to glucocorticoids may underlie variability of clinical efficacy in the vocal folds. Sci Rep 10, 20458
33235235   Curated Info

3

Lea S, et al. (2020) P38 MAPK and glucocorticoid receptor crosstalk in bronchial epithelial cells. J Mol Med (Berl)
31974640   Curated Info

4

Nicoll JX, Fry AC, Mosier EM (2019) Sex-based differences in resting MAPK, androgen, and glucocorticoid receptor phosphorylation in human skeletal muscle. Steroids 141, 23-29
30414425   Curated Info

5

Qattan MY, et al. (2017) Differential regulation of cell death pathways by the microenvironment correlates with chemoresistance and survival in leukaemia. PLoS One 12, e0178606
28582465   Curated Info

6

Matthews LC, et al. (2015) Glucocorticoid receptor regulates accurate chromosome segregation and is associated with malignancy. Proc Natl Acad Sci U S A 112, 5479-84
25847991   Curated Info

7

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

8

Bouazza B, et al. (2014) Basal p38 Mitogen-Activated Protein Kinase Regulates Unliganded Glucocorticoid Receptor Function in Airway Smooth Muscle Cells. Am J Respir Cell Mol Biol 50, 301-15
24024586   Curated Info

9

Galliher-Beckley AJ, Williams JG, Cidlowski JA (2011) Ligand-independent phosphorylation of the glucocorticoid receptor integrates cellular stress pathways with nuclear receptor signaling. Mol Cell Biol 31, 4663-75
21930780   Curated Info

10

Matthews L, et al. (2011) Cell cycle phase regulates glucocorticoid receptor function. PLoS One 6, e22289
21829454   Curated Info

11

Avenant C, Kotitschke A, Hapgood JP (2010) Glucocorticoid receptor phosphorylation modulates transcription efficacy through GRIP-1 recruitment. Biochemistry 49, 972-85
20047289   Curated Info

12

Garza AM, Khan SH, Kumar R (2010) Site-specific phosphorylation induces functionally active conformation in the intrinsically disordered N-terminal activation function (AF1) domain of the glucocorticoid receptor. Mol Cell Biol 30, 220-30
19841061   Curated Info

13

Lynch JT, et al. (2010) The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression. Mol Cancer 9, 38
20156337   Curated Info

14

Kotitschke A, et al. (2009) Genomic and nongenomic cross talk between the gonadotropin-releasing hormone receptor and glucocorticoid receptor signaling pathways. Mol Endocrinol 23, 1726-45
19812390   Curated Info

15

Galliher-Beckley AJ, Williams JG, Collins JB, Cidlowski JA (2008) Glycogen Synthase Kinase 3{beta}-Mediated Serine Phosphorylation of the Human Glucocorticoid Receptor Redirects Gene Expression Profiles. Mol Cell Biol 28, 7309-22
18838540   Curated Info

16

Kino T, et al. (2007) Cyclin-dependent kinase 5 differentially regulates the transcriptional activity of the glucocorticoid receptor through phosphorylation: clinical implications for the nervous system response to glucocorticoids and stress. Mol Endocrinol 21, 1552-68
17440046   Curated Info

17

Wang Z, et al. (2007) Modulation of glucocorticoid receptor phosphorylation and transcriptional activity by a C-terminal-associated protein phosphatase. Mol Endocrinol 21, 625-34
17185395   Curated Info

18

Miller AL, Garza AS, Johnson BH, Thompson EB (2007) Pathway interactions between MAPKs, mTOR, PKA, and the glucocorticoid receptor in lymphoid cells. Cancer Cell Int 7, 3
17391526   Curated Info

19

Koubovec D, et al. (2005) Synthetic progestins used in HRT have different glucocorticoid agonist properties. Mol Cell Endocrinol 242, 23-32
16125839   Curated Info

20

Miller AL, et al. (2005) p38 Mitogen-activated protein kinase (MAPK) is a key mediator in glucocorticoid-induced apoptosis of lymphoid cells: correlation between p38 MAPK activation and site-specific phosphorylation of the human glucocorticoid receptor at serine 211. Mol Endocrinol 19, 1569-83
15817653   Curated Info

21

Wang Z, Frederick J, Garabedian MJ (2002) Deciphering the phosphorylation "code" of the glucocorticoid receptor in vivo. J Biol Chem 277, 26573-80
12000743   Curated Info