Ser203
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Home > Phosphorylation Site Page: > Ser203  -  GR (human)

Site Information
DLEFSSGsPGkEtNE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447967

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 7 ) , immunoprecipitation ( 7 , 11 ) , mass spectrometry ( 3 , 5 , 6 , 9 ) , mutation of modification site ( 4 , 7 , 8 , 10 , 11 , 12 ) , phospho-antibody ( 1 , 2 , 4 , 7 , 11 , 12 ) , western blotting ( 1 , 2 , 4 , 7 , 12 )
Disease tissue studied:
bone cancer ( 12 ) , lung cancer ( 6 , 12 ) , non-small cell lung cancer ( 6 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 4 ) , 293 (epithelial) ( 7 ) , A549 (pulmonary) ( 12 ) , COS (fibroblast) ( 8 ) , fibroblast ( 1 ) , H2009 (pulmonary) ( 6 ) , H2887 (pulmonary) ( 6 ) , HCC2279 (pulmonary) ( 6 ) , HCT116 (intestinal) ( 10 ) , HeLa (cervical) ( 2 , 3 , 7 ) , Jurkat (T lymphocyte) ( 9 ) , liver ( 5 , 12 ) , lymph node ( 12 ) , NCI-H2172 (pulmonary) ( 6 ) , prostate ( 12 ) , thyroid ( 12 ) , U2OS (bone cell) [GR (human)] ( 11 , 12 )

Upstream Regulation
Regulatory protein:
MKK3 (human) ( 4 ) , PPP5C (human) ( 4 )
Putative in vivo kinases:
CDK5 (human) ( 10 ) , P38A (human) ( 4 )
Kinases, in vitro:
CDK5 (human) ( 10 ) , P38A (human) ( 4 )
Putative upstream phosphatases:
PPP5C (human) ( 11 )
Treatments:
aphidicolin ( 7 ) , dexamethasone ( 1 , 11 , 12 ) , fluocinolone ( 12 ) , fluticasone ( 4 ) , geldanamycin ( 11 ) , hydroxyurea ( 7 ) , Methylprednisolone ( 1 ) , mifepristone ( 12 ) , nocodazole ( 7 ) , prednisolone ( 12 ) , SB203580 ( 4 ) , seliciclib ( 7 ) , siRNA ( 4 ) , taxol ( 7 ) , triamcinolone ( 1 ) , VX-680 ( 2 ) , ZK299 ( 12 )

Downstream Regulation
Effects of modification on GR:
activity, inhibited ( 10 ) , intracellular localization ( 2 , 4 ) , molecular association, regulation ( 8 ) , phosphorylation ( 4 )
Effects of modification on biological processes:
transcription, induced ( 8 ) , transcription, inhibited ( 4 , 10 )
Induce interaction with:
GRIP1 (human) ( 8 )

References 

1

Nakamura R, et al. (2020) Complex fibroblast response to glucocorticoids may underlie variability of clinical efficacy in the vocal folds. Sci Rep 10, 20458
33235235   Curated Info

2

Matthews LC, et al. (2015) Glucocorticoid receptor regulates accurate chromosome segregation and is associated with malignancy. Proc Natl Acad Sci U S A 112, 5479-84
25847991   Curated Info

3

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

4

Bouazza B, et al. (2014) Basal p38 Mitogen-Activated Protein Kinase Regulates Unliganded Glucocorticoid Receptor Function in Airway Smooth Muscle Cells. Am J Respir Cell Mol Biol 50, 301-15
24024586   Curated Info

5

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

6

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

7

Matthews L, et al. (2011) Cell cycle phase regulates glucocorticoid receptor function. PLoS One 6, e22289
21829454   Curated Info

8

Avenant C, Kotitschke A, Hapgood JP (2010) Glucocorticoid receptor phosphorylation modulates transcription efficacy through GRIP-1 recruitment. Biochemistry 49, 972-85
20047289   Curated Info

9

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

10

Kino T, et al. (2007) Cyclin-dependent kinase 5 differentially regulates the transcriptional activity of the glucocorticoid receptor through phosphorylation: clinical implications for the nervous system response to glucocorticoids and stress. Mol Endocrinol 21, 1552-68
17440046   Curated Info

11

Wang Z, et al. (2007) Modulation of glucocorticoid receptor phosphorylation and transcriptional activity by a C-terminal-associated protein phosphatase. Mol Endocrinol 21, 625-34
17185395   Curated Info

12

Wang Z, Frederick J, Garabedian MJ (2002) Deciphering the phosphorylation "code" of the glucocorticoid receptor in vivo. J Biol Chem 277, 26573-80
12000743   Curated Info