Lys320
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Home > Acetylation Site Page: > Lys320  -  p53 (human)

Site Information
sssPQPkkkPLDGEy   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 458976

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 6 , 13 , 23 ) , immunoprecipitation ( 10 , 17 , 18 , 22 , 33 , 36 , 37 , 38 , 41 ) , mass spectrometry (in vitro) ( 42 ) , modification-specific antibody ( 6 , 8 , 10 , 13 , 14 , 18 , 19 , 20 , 23 , 28 , 32 , 33 , 34 , 39 , 41 ) , mutation of modification site ( 7 , 12 , 13 , 16 , 17 , 20 , 22 , 23 , 28 , 30 , 31 , 32 , 33 , 41 ) , phospho-antibody ( 4 , 20 , 32 , 36 , 39 , 42 ) , western blotting ( 4 , 6 , 8 , 10 , 13 , 14 , 17 , 18 , 19 , 20 , 22 , 23 , 28 , 32 , 33 , 34 , 36 , 37 , 38 , 39 , 41 , 42 )
Disease tissue studied:
ataxia-telangiectasia ( 36 ) , adrenal cancer ( 34 ) , pheochromocytoma ( 34 ) , bone cancer ( 17 , 18 , 22 , 41 ) , colorectal cancer ( 4 , 16 , 19 , 30 , 32 , 33 , 42 ) , colorectal carcinoma ( 4 , 16 , 19 , 30 , 32 , 33 , 42 ) , kidney cancer ( 38 ) , leukemia ( 34 ) , T cell leukemia ( 34 ) , lung cancer ( 7 , 12 , 20 , 22 , 23 , 28 , 30 , 32 , 34 , 36 , 37 , 42 ) , non-small cell lung cancer ( 7 , 12 , 20 , 22 , 23 , 28 , 30 , 32 , 37 , 42 ) , non-small cell lung adenocarcinoma ( 20 , 34 , 37 , 42 ) , neuroblastoma ( 34 ) , prostate cancer ( 10 ) , testicular cancer ( 23 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
E1B, small T-antigen (adenovirus) ( 38 ) , ID4 (human) ( 10 ) , p53 (human) ( 36 ) , PTMA (human) ( 28 ) , TAT (human) ( 34 ) , ZBTB8 (human) ( 8 )
Treatments:
5-aza-CdR ( 20 ) , adriamycin ( 14 , 32 ) , ALLN ( 42 ) , deferoxamine ( 18 ) , etoposide ( 18 ) , H2O2 ( 19 ) , ionizing_radiation ( 32 , 36 , 41 , 42 ) , laminar flow ( 33 ) , nocodazole ( 32 ) , PALA ( 32 ) , plumbagin ( 14 ) , retinoic_acid ( 23 ) , taxol ( 32 ) , trichostatin_A ( 6 , 19 , 29 , 33 , 38 ) , UV ( 32 , 33 , 34 , 36 , 39 , 41 ) , UVC ( 42 ) , virus infection ( 34 )

Downstream Regulation
Effects of modification on p53:
activity, induced ( 41 ) , intracellular localization ( 12 ) , molecular association, regulation ( 8 , 10 , 20 , 22 , 23 , 28 , 34 , 38 , 41 , 42 ) , protein stabilization ( 37 )
Effects of modification on biological processes:
apoptosis, induced ( 16 , 18 ) , cell growth, induced ( 13 , 20 , 23 ) , neural plasticity ( 13 ) , transcription, induced ( 8 , 10 , 13 , 18 , 19 , 22 , 30 , 34 , 38 , 41 ) , transcription, inhibited ( 24 , 33 )
Induce interaction with:
DNA ( 8 , 10 , 20 , 22 , 23 , 28 , 34 , 38 , 41 , 42 )

References 

1

Head PE, et al. (2017) Sirtuin 2 mutations in human cancers impair its function in genome maintenance. J Biol Chem 292, 9919-9931
28461331   Curated Info

2

Ryu HW, et al. (2017) HDAC6 deacetylates p53 at lysines 381/382 and differentially coordinates p53-induced apoptosis. Cancer Lett 391, 162-171
28153791   Curated Info

3

Choi OR, Ryu MS, Lim IK (2016) Shifting p53-induced senescence to cell death by TIS21(/BTG2/Pc3) gene through posttranslational modification of p53 protein. Cell Signal 28, 1172-85
27208501   Curated Info

4

Sen N, Kumari R, Singh MI, Das S (2013) HDAC5, a key component in temporal regulation of p53-mediated transactivation in response to genotoxic stress. Mol Cell 52, 406-20
24120667   Curated Info

5

Kim SS, Benchimol S (2013) HDAC5--a critical player in the p53 acetylation network. Mol Cell 52, 289-90
24207022   Curated Info

6

Brochier C, et al. (2013) Specific acetylation of p53 by HDAC inhibition prevents DNA damage-induced apoptosis in neurons. J Neurosci 33, 8621-32
23678107   Curated Info

7

van Leeuwen IM, et al. (2013) Modulation of p53 C-Terminal Acetylation by mdm2, p14ARF, and Cytoplasmic SirT2. Mol Cancer Ther 12, 471-80
23416275   Curated Info

8

Kim MK, et al. (2013) Regulation of the Cyclin-dependent Kinase Inhibitor 1A Gene (CDKN1A) by the Repressor BOZF1 through Inhibition of p53 Acetylation and Transcription Factor Sp1 Binding. J Biol Chem 288, 7053-64
23329847   Curated Info

9

Chan C, et al. (2013) Altered Binding Site Selection of p53 Transcription Cassettes by Hepatitis B Virus X Protein. Mol Cell Biol 33, 485-97
23149944   Curated Info

10

Knowell AE, et al. (2013) Id4 dependent acetylation restores mutant-p53 transcriptional activity. Mol Cancer 12, 161
24330748   Curated Info

11

Seo SK, et al. (2011) Histone deacetylase inhibitors sensitize human non-small cell lung cancer cells to ionizing radiation through acetyl p53-mediated c-myc down-regulation. J Thorac Oncol 6, 1313-9
21642861   Curated Info

12

Kuroda T, et al. (2011) RNA content in the nucleolus alters p53 acetylation via MYBBP1A. EMBO J 30, 1054-66
21297583   Curated Info

13

Gaub P, et al. (2010) HDAC inhibition promotes neuronal outgrowth and counteracts growth cone collapse through CBP/p300 and P/CAF-dependent p53 acetylation. Cell Death Differ 17, 1392-408
20094059   Curated Info

14

Ravindra KC, et al. (2009) Inhibition of lysine acetyltransferase KAT3B/p300 activity by a naturally occurring hydroxynaphthoquinone, plumbagin. J Biol Chem 284, 24453-64
19570987   Curated Info

15

Wu SY, Chiang CM (2009) Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding. EMBO J 28, 1246-59
19339993   Curated Info

16

Yamaguchi H, et al. (2009) p53 Acetylation Is Crucial for Its Transcription-independent Proapoptotic Functions. J Biol Chem 284, 11171-83
19265193   Curated Info

17

Yang W, Wetterskog D, Matsumoto Y, Funa K (2008) Kinetics of repression by modified p53 on the PDGF beta-receptor promoter. Int J Cancer 123, 2020-30
18697203   Curated Info

18

Xenaki G, et al. (2008) PCAF is an HIF-1alpha cofactor that regulates p53 transcriptional activity in hypoxia. Oncogene 27, 5785-96
18574470   Curated Info

19

Habold C, et al. (2008) Trichostatin A causes p53 to switch oxidative-damaged colorectal cancer cells from cell cycle arrest into apoptosis. J Cell Mol Med 12, 607-21
18419600   Curated Info

20

Wang H, et al. (2008) An ATM- and Rad3-related (ATR) Signaling Pathway and a Phosphorylation-Acetylation Cascade Are Involved in Activation of p53/p21Waf1/Cip1 in Response to 5-Aza-2'-deoxycytidine Treatment. J Biol Chem 283, 2564-74
17977830   Curated Info

21

Higashitsuji H, et al. (2007) Enhanced deacetylation of p53 by the anti-apoptotic protein HSCO in association with histone deacetylase 1. J Biol Chem 282, 13716-25
17353187   Curated Info

22

Sasaki T, et al. (2007) HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53. Genes Dev 21, 848-61
17403783   Curated Info

23

Di Giovanni S, et al. (2006) The tumor suppressor protein p53 is required for neurite outgrowth and axon regeneration. EMBO J 25, 4084-96
16946709   Curated Info

24

Wu SY, et al. (2006) Brd4 links chromatin targeting to HPV transcriptional silencing. Genes Dev 20, 2383-96
16921027   Curated Info

25

Li AG, et al. (2006) Mechanistic insights into maintenance of high p53 acetylation by PTEN. Mol Cell 23, 575-87
16916644   Curated Info

26

Knights CD, et al. (2006) Distinct p53 acetylation cassettes differentially influence gene-expression patterns and cell fate. J Cell Biol 173, 533-44
16717128   Curated Info

27

Moiseeva O, et al. (2006) DNA damage signaling and p53-dependent senescence after prolonged beta-interferon stimulation. Mol Biol Cell 17, 1583-92
16436515   Curated Info

28

Kobayashi T, et al. (2006) Overexpression of the oncoprotein prothymosin alpha triggers a p53 response that involves p53 acetylation. Cancer Res 66, 3137-44
16540664   Curated Info

29

Roy S, Packman K, Jeffrey R, Tenniswood M (2005) Histone deacetylase inhibitors differentially stabilize acetylated p53 and induce cell cycle arrest or apoptosis in prostate cancer cells. Cell Death Differ 12, 482-91
15746940   Curated Info

30

Hsu CH, et al. (2004) HCMV IE2-mediated inhibition of HAT activity downregulates p53 function. EMBO J 23, 2269-80
15141169   Curated Info

31

Mujtaba S, et al. (2004) Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation. Mol Cell 13, 251-63
14759370   Curated Info

32

Saito S, et al. (2003) Phosphorylation site interdependence of human p53 post-translational modifications in response to stress. J Biol Chem 278, 37536-44
12860987   Curated Info

33

Zeng L, et al. (2003) The role of p53 deacetylation in p21Waf1 regulation by laminar flow. J Biol Chem 278, 24594-9
12716906   Curated Info

34

Harrod R, et al. (2003) Human immunodeficiency virus type-1 Tat/co-activator acetyltransferase interactions inhibit p53Lys-320 acetylation and p53-responsive transcription. J Biol Chem 278, 12310-8
12501250   Curated Info

35

Ito A, et al. (2002) MDM2-HDAC1-mediated deacetylation of p53 is required for its degradation. EMBO J 21, 6236-45
12426395   Curated Info

36

Saito S, et al. (2002) ATM mediates phosphorylation at multiple p53 sites, including Ser(46), in response to ionizing radiation. J Biol Chem 277, 12491-4
11875057   Curated Info

37

Ito A, et al. (2001) p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2. EMBO J 20, 1331-40
11250899   Curated Info

38

Liu Y, Colosimo AL, Yang XJ, Liao D (2000) Adenovirus E1B 55-kilodalton oncoprotein inhibits p53 acetylation by PCAF. Mol Cell Biol 20, 5540-53
10891493   Curated Info

39

Buschmann T, et al. (2000) p53 phosphorylation and association with murine double minute 2, c-Jun NH2-terminal kinase, p14ARF, and p300/CBP during the cell cycle and after exposure to ultraviolet irradiation. Cancer Res 60, 896-900
10706102   Curated Info

40

Nishimori H, et al. (2000) Analysis of the p300/CBP-Associated Factor (PCAF) gene in astrocytic tumors. J Neurooncol 46, 17-22
10896202   Curated Info

41

Liu L, et al. (1999) p53 sites acetylated in vitro by PCAF and p300 are acetylated in vivo in response to DNA damage. Mol Cell Biol 19, 1202-9
9891054   Curated Info

42

Sakaguchi K, et al. (1998) DNA damage activates p53 through a phosphorylation-acetylation cascade. Genes Dev 12, 2831-41
9744860   Curated Info