Lys49
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Home > Acetylation Site Page: > Lys49  -  CTNNB1 (human)

Site Information
TAPsLsGkGNPEEED   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 458938

In vivo Characterization
Methods used to characterize site in vivo:
[14C] acetate labeling ( 15 ) , flow cytometry ( 2 , 5 ) , immunoprecipitation ( 1 , 3 , 4 , 5 , 6 , 8 , 10 , 14 , 15 ) , microscopy-colocalization with upstream kinase ( 6 ) , modification-specific antibody ( 4 , 10 , 11 , 12 , 13 , 14 ) , mutation of modification site ( 1 , 6 , 10 , 14 , 15 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 8 , 10 , 11 , 12 , 13 , 14 , 15 )
Disease tissue studied:
bone cancer ( 13 ) , brain cancer ( 8 ) , glioblastoma ( 8 ) , glioma ( 8 ) , colorectal cancer ( 3 , 5 , 10 , 11 ) , colorectal carcinoma ( 3 , 5 , 10 , 11 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:
'stem, embryonic' ( 10 ) , 293 (epithelial) ( 6 , 10 , 15 ) , A549 (pulmonary) ( 2 ) , brain ( 6 ) , Calu-1 (squamous) ( 2 ) , E.coli (bacterial) ( 11 ) , embryonic ( 4 ) , HCT116 (intestinal) ( 3 , 5 ) , HEK293T (epithelial) ( 2 , 3 , 5 , 11 , 14 , 15 ) , HeLa (cervical) ( 15 ) , LoVo (intestinal) ( 5 ) , NCI-H1299 (pulmonary) ( 2 ) , neuron:neurosphere-brain ( 10 ) , NPC (neural crest) ( 12 ) , NRK49F (fibroblast) ( 1 ) , SHG44 (glial) ( 8 ) , SK-LU-1 (pulmonary) ( 2 ) , SW480 (intestinal) ( 5 , 10 , 11 ) , SW620 (intestinal) ( 5 ) , U-118MG (glial) ( 8 ) , U-251MG (glioma) ( 8 ) , U2OS (bone cell) ( 13 ) , U87MG (glial) ( 8 )

Upstream Regulation
Regulatory protein:
Bcl-3 (human) ( 5 ) , DBC1 (human) ( 11 ) , HDAC6 (human) ( 16 ) , HDAC7 (human) ( 2 ) , PKCA (human) ( 14 ) , ROCK1 (human) ( 13 ) , ZNF326 (human) ( 8 )
Treatments:
ACY1215 ( 12 ) , anacardic acid ( 10 ) , BG-45 ( 12 ) , CI994 ( 12 ) , CPD-60 ( 12 ) , crebinostat ( 12 ) , EGF ( 16 ) , siRNA ( 12 , 16 ) , trichostatin_A ( 13 ) , tubacin ( 12 ) , Tubastatin A ( 12 ) , virus infection ( 14 ) , vorinostat ( 12 ) , Wnt3a ( 5 ) , Y27632 ( 13 )

Downstream Regulation
Effects of modification on CTNNB1:
acetylation ( 5 ) , intracellular localization ( 2 , 6 , 8 , 12 , 14 , 15 , 16 ) , molecular association, regulation ( 2 , 5 , 11 ) , phosphorylation ( 6 ) , protein stabilization ( 6 , 13 , 15 ) , ubiquitination ( 6 , 12 )
Effects of modification on biological processes:
apoptosis, inhibited ( 6 ) , carcinogenesis, induced ( 3 ) , carcinogenesis, inhibited ( 15 ) , cell differentiation, induced ( 4 , 10 ) , cell growth, induced ( 3 ) , cell motility, induced ( 15 ) , transcription, induced ( 3 , 4 , 5 , 6 , 10 , 11 , 15 ) , transcription, inhibited ( 8 , 14 )
Induce interaction with:
Bcl-3 (human) ( 5 ) , HDAC7 (human) ( 2 ) , LEF-1 (human) ( 11 )

References 

1

Chen DQ, et al. (2023) Poricoic acid A suppresses renal fibroblast activation and interstitial fibrosis in UUO rats via upregulating Sirt3 and promoting β-catenin K49 deacetylation. Acta Pharmacol Sin 44, 1038-1050
36470978   Curated Info

2

Guo K, et al. (2022) HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway. J Exp Clin Cancer Res 41, 91
35277183   Curated Info

3

Ning J, et al. (2022) The CK1δ/ϵ-Tip60 Axis Enhances Wnt/β-Catenin Signaling Regulating β-Catenin Acetylation in Colon Cancer. Front Oncol 12, 844477
35494070   Curated Info

4

Oginuma M, et al. (2020) Intracellular pH controls WNT downstream of glycolysis in amniote embryos. Nature 584, 98-101
32581357   Curated Info

5

Chen X, et al. (2020) Bcl-3 promotes Wnt signaling by maintaining the acetylation of β-catenin at lysine 49 in colorectal cancer. Signal Transduct Target Ther 5, 52
32355204   Curated Info

6

Liu E, et al. (2020) Tau acetylates and stabilizes β-catenin thereby promoting cell survival. EMBO Rep 21, e48328
31930681   Curated Info

7

El Ayachi I, et al. (2019) The WNT10B Network Is Associated with Survival and Metastases in Chemoresistant Triple-Negative Breast Cancer. Cancer Res 79, 982-993
30563890   Curated Info

8

Yu X, et al. (2019) ZNF326 promotes malignant phenotype of glioma by up-regulating HDAC7 expression and activating Wnt pathway. J Exp Clin Cancer Res 38, 40
30691485   Curated Info

9

Shares BH, et al. (2018) Active mitochondria support osteogenic differentiation by stimulating ¿¿-catenin acetylation. J Biol Chem
30150300   Curated Info

10

Hoffmeyer K, Junghans D, Kanzler B, Kemler R (2017) Trimethylation and Acetylation of β-Catenin at Lysine 49 Represent Key Elements in ESC Pluripotency. Cell Rep 18, 2815-2824
28329675   Curated Info

11

Yu EJ, et al. (2016) Positive regulation of β-catenin-PROX1 signaling axis by DBC1 in colon cancer progression. Oncogene 35, 3410-8
26477307   Curated Info

12

Iaconelli J, et al. (2015) HDAC6 inhibitors modulate Lys49 acetylation and membrane localization of β-catenin in human iPSC-derived neuronal cells. ACS Chem Biol 10, 883-90
25546293   Curated Info

13

Schofield AV, Gamell C, Bernard O (2013) Tubulin polymerization promoting protein 1 (TPPP1) increases β-catenin expression through inhibition of HDAC6 activity in U2OS osteosarcoma cells. Biochem Biophys Res Commun 436, 571-7
23727580   Curated Info

14

Zhu J, Coyne CB, Sarkar SN (2011) PKC alpha regulates Sendai virus-mediated interferon induction through HDAC6 and β-catenin. EMBO J 30, 4838-49
21952047   Curated Info

15

Ge X, et al. (2009) PCAF acetylates {beta}-catenin and improves its stability. Mol Biol Cell 20, 419-27
18987336   Curated Info

16

Li Y, et al. (2008) HDAC6 is required for epidermal growth factor-induced beta-catenin nuclear localization. J Biol Chem 283, 12686-90
18356165   Curated Info

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