Ser73
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Home > Phosphorylation Site Page: > Ser73  -  Jun (rat)

Site Information
VGLLKLAsPELERLI   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447943

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 12 , 13 ) , flow cytometry ( 8 ) , immunoprecipitation ( 4 , 12 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 5 , 12 , 13 ) , phospho-antibody ( 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ) , phosphopeptide mapping ( 13 ) , western blotting ( 4 , 5 , 7 , 8 , 9 , 10 )
Disease tissue studied:
adrenal cancer ( 7 ) , pheochromocytoma ( 7 ) , brain cancer ( 4 ) , glioblastoma ( 4 ) , glioma ( 4 ) , ovarian cancer ( 4 ) , testicular cancer ( 13 )
Relevant cell line - cell type - tissue:
'neuron, superior cervical ganglion'-brain ( 6 ) , 'neuron, sympathetic' ( 5 ) , 208F ( 12 ) , 3T3 (fibroblast) ( 9 ) , adipose tissue ( 2 ) , F9 (testicular) ( 13 ) , Fr 3T3 (fibroblast) ( 13 ) , heart ( 2 , 11 ) , HEK293T (epithelial) ( 4 ) , IEC-6 (epithelial) ( 1 ) , intestine ( 2 ) , liver ( 2 , 3 ) , lung ( 2 ) , MEF (fibroblast) ( 13 ) , myocyte ( 10 ) , myocyte-heart ( 8 ) , OVCAR5 (ovarian) ( 4 ) , PC-12 (chromaffin) ( 7 ) , Rat1 (fibroblast) ( 9 ) , SNB-19 (glial) ( 4 ) , stomach ( 2 ) , thymus ( 2 )

Upstream Regulation
Regulatory protein:
AMD1 (mouse) ( 9 ) , HRas (mouse) ( 13 ) , JNK3 (rat) ( 7 )
Kinases, in vitro:
CDK1 (human) ( 12 ) , CK2A1 (rat) ( 12 ) , JNK1 (rat) ( 11 )
Treatments:
benzodiazepinones ( 4 ) , CEP1347 ( 5 ) , endothelin ( 8 ) , GDNF_withdrawal ( 6 ) , GF109203X ( 8 ) , ischemia/reperfusion ( 11 ) , NGF ( 7 ) , NGF_withdrawal ( 5 , 6 ) , PD98059 ( 8 ) , phenylephrine ( 8 ) , phorbol_ester ( 8 ) , SB203580 ( 8 ) , serum ( 12 ) , sorbitol ( 8 )

Downstream Regulation
Effects of modification on Jun:
activity, induced ( 13 ) , protein stabilization ( 8 )
Effects of modification on biological processes:
cell growth, altered ( 13 ) , transcription, altered ( 13 )

References 

1

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

4

Kim I, et al. (2009) Chemical biology investigation of cell death pathways activated by endoplasmic reticulum stress reveals cytoprotective modulators of ASK1. J Biol Chem 284, 1593-603
19004820   Curated Info

5

Besirli CG, Wagner EF, Johnson EM (2005) The limited role of NH2-terminal c-Jun phosphorylation in neuronal apoptosis: identification of the nuclear pore complex as a potential target of the JNK pathway. J Cell Biol 170, 401-11
16061693   Curated Info

6

Yu LY, et al. (2003) GDNF-deprived sympathetic neurons die via a novel nonmitochondrial pathway. J Cell Biol 163, 987-97
14657232   Curated Info

7

Waetzig V, Herdegen T (2003) A single c-Jun N-terminal kinase isoform (JNK3-p54) is an effector in both neuronal differentiation and cell death. J Biol Chem 278, 567-72
12401814   Curated Info

8

Clerk A, et al. (2002) Up-regulation of c-jun mRNA in cardiac myocytes requires the extracellular signal-regulated kinase cascade, but c-Jun N-terminal kinases are required for efficient up-regulation of c-Jun protein. Biochem J 368, 101-10
12169099   Curated Info

9

Paasinen-Sohns A, et al. (2000) c-Jun activation-dependent tumorigenic transformation induced paradoxically by overexpression or block of S-adenosylmethionine decarboxylase. J Cell Biol 151, 801-10
11076965   Curated Info

10

Clerk A, Sugden PH (1997) Cell stress-induced phosphorylation of ATF2 and c-Jun transcription factors in rat ventricular myocytes. Biochem J 325 ( Pt 3), 801-10
9271103   Curated Info

11

Mizukami Y, Yoshioka K, Morimoto S, Yoshida K (1997) A novel mechanism of JNK1 activation. Nuclear translocation and activation of JNK1 during ischemia and reperfusion. J Biol Chem 272, 16657-62
9195981   Curated Info

12

Baker SJ, et al. (1992) Jun is phosphorylated by several protein kinases at the same sites that are modified in serum-stimulated fibroblasts. Mol Cell Biol 12, 4694-705
1328860   Curated Info

13

Smeal T, et al. (1991) Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73. Nature 354, 494-6
1749429   Curated Info