Lys323
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Home > Acetylation Site Page: > Lys323  -  Myc (human)

Site Information
RKDYPAAkRVKLDSV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 458888

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 3 ) , mass spectrometry ( 2 , 4 , 5 , 6 , 7 , 8 , 9 ) , mass spectrometry (in vitro) ( 9 , 10 ) , modification-specific antibody ( 1 , 3 ) , mutation of modification site ( 1 , 9 ) , western blotting ( 1 , 3 , 9 )
Disease tissue studied:
breast cancer ( 1 ) , colorectal cancer ( 4 , 5 , 6 , 7 ) , colorectal carcinoma ( 4 , 5 , 6 , 7 ) , leukemia ( 1 , 3 ) , acute myelogenous leukemia ( 1 , 3 ) , chronic myelogenous leukemia ( 1 ) , lymphoma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Treatments:
MS275 ( 1 ) , selisistat ( 1 ) , vorinostat ( 1 )

Downstream Regulation
Effects of modification on Myc:
molecular association, regulation ( 10 )
Effects of modification on biological processes:
transcription, induced ( 1 )
Induce interaction with:
ZBTB17 (human) ( 10 )

References 

1

Nebbioso A, et al. (2017) c-Myc Modulation and Acetylation Is a Key HDAC Inhibitor Target in Cancer. Clin Cancer Res 23, 2542-2555
27358484   Curated Info

2

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

3

Lynch JT, et al. (2013) TTC5 is required to prevent apoptosis of acute myeloid leukemia stem cells. Cell Death Dis 4, e573
23559008   Curated Info

4

Possemato A (2010) CST Curation Set: 9462; Year: 2010; Biosample/Treatment: cell line, HCT 116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK Antibodies Used to Purify Peptides prior to LCMS: Acetylated-Lysine (Ac-K2-100) Rabbit mAb Cat#: 9814, PTMScan(R) Acetyl-Lys Motif (Ac-K) Immunoaffinity Beads Cat#: 1989
Curated Info

5

Possemato A (2010) CST Curation Set: 8991; Year: 2010; Biosample/Treatment: cell line, HCT 116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK Antibodies Used to Purify Peptides prior to LCMS: Acetylated-Lysine (Ac-K2-100) Rabbit mAb Cat#: 9814, PTMScan(R) Acetyl-Lys Motif (Ac-K) Immunoaffinity Beads Cat#: 1989
Curated Info

6

Possemato A (2010) CST Curation Set: 8996; Year: 2010; Biosample/Treatment: cell line, HCT 116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK Antibodies Used to Purify Peptides prior to LCMS: Acetylated-Lysine (Ac-K2-100) Rabbit mAb Cat#: 9814, PTMScan(R) Acetyl-Lys Motif (Ac-K) Immunoaffinity Beads Cat#: 1989
Curated Info

7

Possemato A (2010) CST Curation Set: 8969; Year: 2010; Biosample/Treatment: cell line, HCT 116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK Antibodies Used to Purify Peptides prior to LCMS: Acetylated-Lysine (Ac-K2-100) Rabbit mAb Cat#: 9814, PTMScan(R) Acetyl-Lys Motif (Ac-K) Immunoaffinity Beads Cat#: 1989
Curated Info

8

Possemato A (2009) CST Curation Set: 8176; Year: 2009; Biosample/Treatment: cell line, HCT116/untreated & calyculin_A; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK Antibodies Used to Purify Peptides prior to LCMS: Acetylated-Lysine (Ac-K2-100) Rabbit mAb Cat#: 9814, PTMScan(R) Acetyl-Lys Motif (Ac-K) Immunoaffinity Beads Cat#: 1989
Curated Info

9

Faiola F, et al. (2005) Dual regulation of c-Myc by p300 via acetylation-dependent control of Myc protein turnover and coactivation of Myc-induced transcription. Mol Cell Biol 25, 10220-34
16287840   Curated Info

10

Zhang K, Faiola F, Martinez E (2005) Six lysine residues on c-Myc are direct substrates for acetylation by p300. Biochem Biophys Res Commun 336, 274-80
16126174   Curated Info