Thr183
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Home > Phosphorylation Site Page: > Thr183  -  MST1 (human)

Site Information
DtMAkRNtVIGTPFW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447925

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 6 , 7 ) , immunoprecipitation ( 4 , 5 , 7 , 9 ) , mass spectrometry ( 12 , 13 , 14 , 15 , 16 ) , mutation of modification site ( 5 , 6 , 18 ) , phospho-antibody ( 1 , 2 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 18 ) , western blotting ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 18 )
Disease tissue studied:
glioblastoma multiforme ( 1 , 5 ) , glioma ( 1 , 5 ) , breast cancer ( 2 , 6 , 9 ) , colorectal cancer ( 6 ) , colorectal carcinoma ( 6 ) , liver cancer ( 4 ) , hepatocellular carcinoma ( 4 ) , lung cancer ( 6 ) , non-small cell lung cancer ( 6 ) , non-small cell lung adenocarcinoma ( 6 ) , pancreatic cancer ( 9 ) , pancreatic carcinoma ( 9 ) , prostate cancer ( 7 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
Akt1 (human) ( 11 ) , Akt2 (human) ( 11 ) , COL4A1 (human) ( 4 ) , Ezrin (human) ( 8 ) , FGFR4 (human) ( 2 ) , FRMD6 (human) ( 8 ) , LKB1 (human) ( 6 ) , Merlin (human) ( 8 ) , MST1 (human) ( 9 ) , PHLPP (human) ( 9 ) , PHLPP2 (human) ( 9 )
Putative in vivo kinases:
Akt1 (human) ( 7 ) , MST1 (human) ( 2 , 11 , 18 )
Treatments:
Akt_inhibitor_VIII ( 1 ) , BLU9931 ( 2 ) , doxycycline ( 6 ) , glucose_starvation ( 1 ) , KU-0063794 ( 7 ) , LY294002 ( 7 ) , mutation ( 2 ) , NU7026 ( 1 ) , serum ( 2 ) , serum_starvation ( 7 ) , siRNA ( 1 ) , SP600125 ( 10 ) , staurosporine ( 10 ) , temsirolimus ( 7 ) , Y15 ( 4 )

Downstream Regulation
Effects of modification on MST1:
activity, induced ( 1 ) , enzymatic activity, induced ( 2 , 5 , 18 ) , intracellular localization ( 7 , 18 ) , molecular association, regulation ( 5 )
Effects of modification on biological processes:
apoptosis, induced ( 2 ) , signaling pathway regulation ( 1 , 6 )
Induce interaction with:
MOBKL2A (human) ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
ovarian cancer ( 11 ) , prostate cancer ( 7 )

References 

1

Shiga S, et al. (2019) DNA-PKcs is activated under nutrient starvation and activates Akt, MST1, FoxO3a, and NDR1. Biochem Biophys Res Commun
31679687   Curated Info

2

Turunen SP, et al. (2019) FGFR4 phosphorylates MST1 to confer breast cancer cells resistance to MST1/2-dependent apoptosis. Cell Death Differ
30903103   Curated Info

3

Li C, et al. (2017) A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nat Cell Biol 19, 106-119
28114269   Curated Info

4

Wong KF, et al. (2016) Integrin α2β1 inhibits MST1 kinase phosphorylation and activates Yes-associated protein oncogenic signaling in hepatocellular carcinoma. Oncotarget 7, 77683-77695
27765911   Curated Info

5

Tang F, et al. (2014) hMOB3 modulates MST1 apoptotic signaling and supports tumor growth in glioblastoma multiforme. Cancer Res 74, 3779-89
24872389   Curated Info

6

Mohseni M, et al. (2014) A genetic screen identifies an LKB1-MARK signalling axis controlling the Hippo-YAP pathway. Nat Cell Biol 16, 108-17
24362629   Curated Info

7

Collak FK, et al. (2012) Threonine-120 phosphorylation regulated by phosphoinositide-3-kinase/Akt and mammalian target of rapamycin pathway signaling limits the antitumor activity of mammalian sterile 20-like kinase 1. J Biol Chem 287, 23698-709
22619175   Curated Info

8

Angus L, et al. (2012) Willin/FRMD6 expression activates the Hippo signaling pathway kinases in mammals and antagonizes oncogenic YAP. Oncogene 31, 238-50
21666719   Curated Info

9

Qiao M, et al. (2010) Mst1 is an interacting protein that mediates PHLPPs' induced apoptosis. Mol Cell 38, 512-23
20513427   Curated Info

10

Bi W, et al. (2010) c-Jun N-terminal kinase enhances MST1-mediated pro-apoptotic signaling through phosphorylation at serine 82. J Biol Chem 285, 6259-64
20028971   Curated Info

11

Yuan Z, et al. (2010) Phosphoinositide 3-kinase/Akt inhibits MST1-mediated pro-apoptotic signaling through phosphorylation of threonine 120. J Biol Chem 285, 3815-24
19940129   Curated Info

12

Zhou J (2009) CST Curation Set: 7590; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

13

Zhou J (2009) CST Curation Set: 7593; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

14

Zhou J (2009) CST Curation Set: 7592; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

15

Zhou J (2009) CST Curation Set: 7591; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

16

Zhou J (2009) CST Curation Set: 7589; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

17

Praskova M, Xia F, Avruch J (2008) MOBKL1A/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation. Curr Biol 18, 311-21
18328708   Curated Info

18

Lee KK, Yonehara S (2002) Phosphorylation and dimerization regulate nucleocytoplasmic shuttling of mammalian STE20-like kinase (MST). J Biol Chem 277, 12351-8
11805089   Curated Info