Thr308
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Home > Phosphorylation Site Page: > Thr308  -  Akt1 (cow)

Site Information
KDGATMKtFCGTPEY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447856

In vivo Characterization
Methods used to characterize site in vivo:
phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:
'granulosa, luteal' ( 2 ) , BAEC (endothelial) ( 1 , 3 )

Upstream Regulation
Regulatory protein:
IRS1 (rat) ( 1 )
Treatments:
AP21967 ( 4 ) , cAMP_analog ( 3 ) , dinoprost ( 2 ) , fluid_shear_stress ( 3 ) , H-89 ( 3 ) , IGF-1 ( 2 ) , insulin ( 1 ) , LY294002 ( 3 , 4 ) , mPKI ( 3 ) , phorbol_ester ( 1 ) , rapamycin ( 2 ) , staurosporine ( 4 ) , U0126 ( 2 ) , VEGF ( 3 ) , wortmannin ( 2 , 3 )

Downstream Regulation
Effects of modification on Akt1:
enzymatic activity, induced ( 4 ) , intracellular localization ( 4 )

References 

1

Maeno Y, et al. (2012) Inhibition of Insulin Signaling in Endothelial Cells by Protein Kinase C-induced Phosphorylation of p85 Subunit of Phosphatidylinositol 3-Kinase (PI3K). J Biol Chem 287, 4518-30
22158866   Curated Info

2

Arvisais E, et al. (2010) Prostaglandin F2alpha represses IGF-I-stimulated IRS1/phosphatidylinositol-3-kinase/AKT signaling in the corpus luteum: role of ERK and P70 ribosomal S6 kinase. Mol Endocrinol 24, 632-43
20160123   Curated Info

3

Boo YC, et al. (2002) Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism. Am J Physiol Heart Circ Physiol 283, H1819-28
12384459   Curated Info

4

Scheid MP, Marignani PA, Woodgett JR (2002) Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B. Mol Cell Biol 22, 6247-60
12167717   Curated Info