Ser262
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Home > Phosphorylation Site Page: > Ser262  -  ADRB2 (human)

Site Information
GHGLRRssKFcLKEH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447909

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 4 , 10 ) , mutation of modification site ( 12 ) , phospho-antibody ( 1 , 4 , 7 , 8 , 9 , 11 ) , western blotting ( 1 , 4 , 7 , 8 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 , 7 , 8 , 11 , 12 ) , COS (fibroblast) ( 11 ) , EcR293 (epithelial) ( 9 ) , HeLa (cervical) ( 4 ) , M059K (glial) ( 10 ) , myocyte-heart ( 8 )

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 1 )
Treatments:
(R,R')-4'-aminofenoterol ( 3 ) , alprostadil ( 11 ) , carazolol ( 4 ) , carbachol ( 11 ) , colforsin ( 1 , 4 , 11 ) , fenoterol ( 8 ) , H-89 ( 1 , 7 ) , hypertonic_buffer ( 9 ) , IBMX ( 1 ) , ICI-118,551 ( 4 ) , isoproterenol ( 1 , 3 , 4 , 7 , 8 , 9 ) , methoxyfenoterol ( 8 ) , phorbol_ester ( 11 ) , racepinefrine ( 11 ) , U0126 ( 1 )

References 

1

Adachi N, Hess DT, McLaughlin P, Stamler JS (2016) S-Palmitoylation of a Novel Site in the β2-Adrenergic Receptor Associated with a Novel Intracellular Itinerary. J Biol Chem 291, 20232-46
27481942   Curated Info

2

Carr R, et al. (2014) Development and Characterization of Pepducins as Gs-biased Allosteric Agonists. J Biol Chem 289, 35668-84
25395624   Curated Info

3

Woo AY, et al. (2014) Tyrosine 308 is necessary for ligand-directed Gs protein-biased signaling of β2-adrenoceptor. J Biol Chem 289, 19351-63
24831005   Curated Info

4

Gao S, Malbon C, Wang HY (2014) Probing the stoichiometry of β2-adrenergic receptor phosphorylation by targeted mass spectrometry. J Mol Signal 9, 3
24690384   Curated Info

5

Gimenez LE, et al. (2012) Role of receptor-attached phosphates in binding of visual and non-visual arrestins to G protein-coupled receptors. J Biol Chem 287, 9028-40
22275358   Curated Info

6

Nobles KN, et al. (2011) Distinct phosphorylation sites on the β(2)-adrenergic receptor establish a barcode that encodes differential functions of β-arrestin. Sci Signal 4, ra51
21868357   Curated Info

7

Gehret AU, Hinkle PM (2010) Importance of regions outside the cytoplasmic tail of G-protein-coupled receptors for phosphorylation and dephosphorylation. Biochem J 428, 235-45
20345371   Curated Info

8

Woo AY, et al. (2009) Stereochemistry of an agonist determines coupling preference of beta2-adrenoceptor to different G proteins in cardiomyocytes. Mol Pharmacol 75, 158-65
18838481   Curated Info

9

Iyer V, et al. (2006) Differential phosphorylation and dephosphorylation of beta2-adrenoceptor sites Ser262 and Ser355,356. Br J Pharmacol 147, 249-59
16331289   Curated Info

10

Stokes M (2005) CST Curation Set: 992; Year: 2005; Biosample1/Treatment/Isotope: cell line, M059K/UV/L, Biosample2/Treatment/Isotope: cell line, M059K/UV/H; Disease: glioblastoma; SILAC: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS:p[ST]
Curated Info

11

Tran TM, et al. (2004) Characterization of agonist stimulation of cAMP-dependent protein kinase and G protein-coupled receptor kinase phosphorylation of the beta2-adrenergic receptor using phosphoserine-specific antibodies. Mol Pharmacol 65, 196-206
14722251   Curated Info

12

Friedman J, Babu B, Clark RB (2002) Beta(2)-adrenergic receptor lacking the cyclic AMP-dependent protein kinase consensus sites fully activates extracellular signal-regulated kinase 1/2 in human embryonic kidney 293 cells: lack of evidence for G(s)/G(i) switching. Mol Pharmacol 62, 1094-102
12391272   Curated Info

13

Hebert TE, et al. (1998) Functional rescue of a constitutively desensitized beta2AR through receptor dimerization. Biochem J 330 ( Pt 1), 287-93
9461522   Curated Info