Ser520
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Home > Phosphorylation Site Page: > Ser520  -  ZAP70 (human)

Site Information
FRKFSSRsDVWSYGV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2861415

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 2 ) , mass spectrometry ( 1 ) , mutation of modification site ( 2 ) , peptide sequencing ( 2 ) , phosphoamino acid analysis ( 2 ) , phosphopeptide mapping ( 2 )
Relevant cell line - cell type - tissue:

Downstream Regulation
Effects of modification on ZAP70:
intracellular localization ( 2 ) , phosphorylation ( 2 )
Effects of modification on biological processes:
transcription, altered ( 2 )

References 

1

Possemato A (2008) CST Curation Set: 3726; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

Yang Y, Villain P, Mustelin T, Couture C (2003) Critical role of Ser-520 phosphorylation for membrane recruitment and activation of the ZAP-70 tyrosine kinase in T cells. Mol Cell Biol 23, 7667-77
14560012   Curated Info