Thr145
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.7
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr145  -  p21Cip1 (human)

Site Information
QGRkRRQtsMTDFyH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447837

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 16 ) , [32P] bio-synthetic labeling ( 10 , 13 , 15 , 16 ) , flow cytometry ( 6 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 6 , 7 , 8 , 10 , 12 , 13 , 14 , 15 , 16 ) , peptide sequencing ( 16 ) , phospho-antibody ( 1 , 4 , 6 , 8 , 10 , 11 , 12 , 16 ) , phosphopeptide mapping ( 15 ) , western blotting ( 1 , 4 , 6 , 8 , 10 , 12 )
Disease tissue studied:
bone cancer ( 10 ) , colorectal cancer ( 6 ) , colorectal carcinoma ( 6 ) , lung cancer ( 8 , 12 ) , non-small cell lung cancer ( 8 , 12 ) , prostate cancer ( 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 7 ) , 293E (epithelial) ( 13 ) , 293T (epithelial) ( 11 , 16 ) , 3T3 (fibroblast) ( 7 , 8 , 10 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 16 ) , A172 (glial) ( 13 ) , breast cell-breast ( 11 ) , COS (fibroblast) ( 8 ) , DF1 (fibroblast) ( 1 ) , HCT116 (intestinal) ( 6 ) , HUVEC (endothelial) ( 14 , 15 ) , MDA-MB-453 (breast cell) ( 16 ) , MEF (fibroblast) [IGF1R (mouse)] ( 16 ) , NCI-H1299 (pulmonary) ( 8 , 12 ) , PC3 (prostate cell) ( 4 ) , T98G (glial) ( 13 ) , U-118MG (glial) ( 13 ) , U2OS (bone cell) ( 10 ) , U87MG (glial) ( 13 ) , Vero (epithelial) ( 1 )

Upstream Regulation
Regulatory protein:
Pim1 (human) ( 8 )
Putative in vivo kinases:
Akt1 (human) ( 8 , 11 , 13 , 15 , 16 ) , DAPK3 (human) ( 12 ) , Pim1 (human) ( 8 ) , Pim2 (human) ( 6 )
Kinases, in vitro:
Akt1 (human) ( 9 , 13 , 15 ) , DAPK3 (human) ( 12 ) , Pim1 (human) ( 8 ) , Pim2 (human) ( 6 )
Treatments:
insulin ( 16 ) , LY294002 ( 8 , 13 , 15 , 16 ) , serum ( 13 , 15 ) , silmitasertib ( 4 ) , taxol ( 13 ) , wortmannin ( 8 )

Downstream Regulation
Effects of modification on p21Cip1:
intracellular localization ( 6 , 11 , 16 ) , molecular association, regulation ( 8 , 9 , 13 , 15 ) , phosphorylation ( 2 ) , protein stabilization ( 6 , 8 , 12 )
Effects of modification on biological processes:
cell cycle regulation ( 6 , 9 , 15 ) , cell growth, inhibited ( 6 )
Inhibit interaction with:
Akt2 (human) ( 9 ) , CDK2 (human) ( 15 ) , CDK4 (human) ( 15 ) , PCNA (human) ( 8 , 13 , 15 )

References 

1

Chiu HC, et al. (2016) Suppression of Vimentin Phosphorylation by the Avian Reovirus p17 through Inhibition of CDK1 and Plk1 Impacting the G2/M Phase of the Cell Cycle. PLoS One 11, e0162356
27603133   Curated Info

2

Nakakido M, et al. (2015) PRMT6 increases cytoplasmic localization of p21CDKN1A in cancer cells through arginine methylation and makes more resistant to cytotoxic agents. Oncotarget 6, 30957-67
26436589   Curated Info

3

Suzuki H, et al. (2013) Lats2 phosphorylates p21/CDKN1A after UV irradiation and regulates apoptosis. J Cell Sci 126, 4358-68
23886938   Curated Info

4

Pierre F, et al. (2011) Pre-clinical characterization of CX-4945, a potent and selective small molecule inhibitor of CK2 for the treatment of cancer. Mol Cell Biochem 356, 37-43
21755459   Curated Info

5

Siddiqui-Jain A, et al. (2010) CX-4945, an orally bioavailable selective inhibitor of protein kinase CK2, inhibits prosurvival and angiogenic signaling and exhibits antitumor efficacy. Cancer Res 70, 10288-98
21159648   Curated Info

6

Wang Z, et al. (2010) Pim-2 phosphorylation of p21(Cip1/WAF1) enhances its stability and inhibits cell proliferation in HCT116 cells. Int J Biochem Cell Biol 42, 1030-8
20307683   Curated Info

7

Densham RM, et al. (2009) MST kinases monitor actin cytoskeletal integrity and signal via c-Jun N-terminal kinase stress-activated kinase to regulate p21Waf1/Cip1 stability. Mol Cell Biol 29, 6380-90
19822666   Curated Info

8

Zhang Y, Wang Z, Magnuson NS (2007) Pim-1 kinase-dependent phosphorylation of p21Cip1/WAF1 regulates its stability and cellular localization in H1299 cells. Mol Cancer Res 5, 909-22
17855660   Curated Info

9

Héron-Milhavet L, et al. (2006) Only Akt1 is required for proliferation, while Akt2 promotes cell cycle exit through p21 binding. Mol Cell Biol 26, 8267-80
16982699   Curated Info

10

Järviluoma A, et al. (2006) Phosphorylation of the cyclin-dependent kinase inhibitor p21Cip1 on serine 130 is essential for viral cyclin-mediated bypass of a p21Cip1-imposed G1 arrest. Mol Cell Biol 26, 2430-40
16508017   Curated Info

11

Xia W, et al. (2004) Phosphorylation/cytoplasmic localization of p21Cip1/WAF1 is associated with HER2/neu overexpression and provides a novel combination predictor for poor prognosis in breast cancer patients. Clin Cancer Res 10, 3815-24
15173090   Curated Info

12

Burch LR, et al. (2004) Phage-peptide display identifies the interferon-responsive, death-activated protein kinase family as a novel modifier of MDM2 and p21WAF1. J Mol Biol 337, 115-28
15001356   Curated Info

13

Li Y, Dowbenko D, Lasky LA (2002) AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival. J Biol Chem 277, 11352-61
11756412   Curated Info

14

Rössig L, et al. (2002) Glycogen synthase kinase-3 couples AKT-dependent signaling to the regulation of p21Cip1 degradation. J Biol Chem 277, 9684-9
11779850   Curated Info

15

Rössig L, et al. (2001) Akt-dependent phosphorylation of p21(Cip1) regulates PCNA binding and proliferation of endothelial cells. Mol Cell Biol 21, 5644-57
11463845   Curated Info

16

Zhou BP, et al. (2001) Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells. Nat Cell Biol 3, 245-52
11231573   Curated Info