Thr145
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Home > Phosphorylation Site Page: > Thr145  -  p21Cip1 (human)

Site Information
QGRKRRQtSMTDFYH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447837

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 17 ) , [32P] ATP in vitro ( 4 ) , [32P] bio-synthetic labeling ( 11 , 14 , 16 , 17 ) , flow cytometry ( 7 ) , immunoassay ( 6 ) , immunoprecipitation ( 2 ) , mutation of modification site ( 4 , 7 , 8 , 9 , 11 , 13 , 14 , 15 , 16 , 17 ) , peptide sequencing ( 17 ) , phospho-antibody ( 1 , 2 , 5 , 6 , 7 , 9 , 11 , 12 , 13 , 17 ) , phosphopeptide mapping ( 16 ) , western blotting ( 1 , 2 , 5 , 6 , 7 , 9 , 11 , 13 )
Disease tissue studied:
bone cancer ( 11 ) , breast cancer ( 6 ) , colorectal cancer ( 7 ) , colorectal carcinoma ( 7 ) , lung cancer ( 9 , 13 ) , non-small cell lung cancer ( 9 , 13 ) , pancreatic cancer ( 6 ) , pancreatic carcinoma ( 6 ) , prostate cancer ( 1 , 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
CTNND1 (human) ( 1 ) , Pim1 (human) ( 9 )
Putative in vivo kinases:
Akt1 (human) ( 1 , 9 , 12 , 14 , 16 , 17 ) , DAPK3 (human) ( 13 ) , Pim1 (human) ( 9 ) , Pim2 (human) ( 7 )
Kinases, in vitro:
Akt1 (human) ( 10 , 14 , 16 ) , DAPK3 (human) ( 13 ) , Pim1 (human) ( 9 ) , Pim2 (human) ( 7 )
Treatments:
EGF ( 1 ) , gefitinib ( 1 ) , insulin ( 17 ) , LY294002 ( 1 , 9 , 14 , 16 , 17 ) , serum ( 14 , 16 ) , silmitasertib ( 5 , 6 ) , taxol ( 14 ) , wortmannin ( 9 )

Downstream Regulation
Effects of modification on p21Cip1:
intracellular localization ( 7 , 12 , 17 ) , molecular association, regulation ( 9 , 10 , 14 , 16 ) , phosphorylation ( 3 ) , protein degradation ( 6 ) , protein stabilization ( 1 , 7 , 9 , 13 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 , 6 ) , cell cycle regulation ( 6 , 7 , 10 , 16 ) , cell growth, induced ( 1 ) , cell growth, inhibited ( 7 ) , cell motility, induced ( 1 )
Inhibit interaction with:
Akt2 (human) ( 10 ) , CDK2 (human) ( 16 ) , CDK4 (human) ( 16 ) , PCNA (human) ( 9 , 14 , 16 )

References 

1

Shen Y, et al. (2021) δ-Catenin Participates in EGF/AKT/p21 Signaling and Induces Prostate Cancer Cell Proliferation and Invasion. Int J Mol Sci 22
34069970   Curated Info

2

Chiu HC, et al. (2016) Suppression of Vimentin Phosphorylation by the Avian Reovirus p17 through Inhibition of CDK1 and Plk1 Impacting the G2/M Phase of the Cell Cycle. PLoS One 11, e0162356
27603133   Curated Info

3

Nakakido M, et al. (2015) PRMT6 increases cytoplasmic localization of p21CDKN1A in cancer cells through arginine methylation and makes more resistant to cytotoxic agents. Oncotarget 6, 30957-67
26436589   Curated Info

4

Suzuki H, et al. (2013) Lats2 phosphorylates p21/CDKN1A after UV irradiation and regulates apoptosis. J Cell Sci 126, 4358-68
23886938   Curated Info

5

Pierre F, et al. (2011) Pre-clinical characterization of CX-4945, a potent and selective small molecule inhibitor of CK2 for the treatment of cancer. Mol Cell Biochem 356, 37-43
21755459   Curated Info

6

Siddiqui-Jain A, et al. (2010) CX-4945, an orally bioavailable selective inhibitor of protein kinase CK2, inhibits prosurvival and angiogenic signaling and exhibits antitumor efficacy. Cancer Res 70, 10288-98
21159648   Curated Info

7

Wang Z, et al. (2010) Pim-2 phosphorylation of p21(Cip1/WAF1) enhances its stability and inhibits cell proliferation in HCT116 cells. Int J Biochem Cell Biol 42, 1030-8
20307683   Curated Info

8

Densham RM, et al. (2009) MST kinases monitor actin cytoskeletal integrity and signal via c-Jun N-terminal kinase stress-activated kinase to regulate p21Waf1/Cip1 stability. Mol Cell Biol 29, 6380-90
19822666   Curated Info

9

Zhang Y, Wang Z, Magnuson NS (2007) Pim-1 kinase-dependent phosphorylation of p21Cip1/WAF1 regulates its stability and cellular localization in H1299 cells. Mol Cancer Res 5, 909-22
17855660   Curated Info

10

Héron-Milhavet L, et al. (2006) Only Akt1 is required for proliferation, while Akt2 promotes cell cycle exit through p21 binding. Mol Cell Biol 26, 8267-80
16982699   Curated Info

11

Järviluoma A, et al. (2006) Phosphorylation of the cyclin-dependent kinase inhibitor p21Cip1 on serine 130 is essential for viral cyclin-mediated bypass of a p21Cip1-imposed G1 arrest. Mol Cell Biol 26, 2430-40
16508017   Curated Info

12

Xia W, et al. (2004) Phosphorylation/cytoplasmic localization of p21Cip1/WAF1 is associated with HER2/neu overexpression and provides a novel combination predictor for poor prognosis in breast cancer patients. Clin Cancer Res 10, 3815-24
15173090   Curated Info

13

Burch LR, et al. (2004) Phage-peptide display identifies the interferon-responsive, death-activated protein kinase family as a novel modifier of MDM2 and p21WAF1. J Mol Biol 337, 115-28
15001356   Curated Info

14

Li Y, Dowbenko D, Lasky LA (2002) AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival. J Biol Chem 277, 11352-61
11756412   Curated Info

15

Rössig L, et al. (2002) Glycogen synthase kinase-3 couples AKT-dependent signaling to the regulation of p21Cip1 degradation. J Biol Chem 277, 9684-9
11779850   Curated Info

16

Rössig L, et al. (2001) Akt-dependent phosphorylation of p21(Cip1) regulates PCNA binding and proliferation of endothelial cells. Mol Cell Biol 21, 5644-57
11463845   Curated Info

17

Zhou BP, et al. (2001) Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced phosphorylation in HER-2/neu-overexpressing cells. Nat Cell Biol 3, 245-52
11231573   Curated Info