Ser146
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Home > Phosphorylation Site Page: > Ser146  -  p21Cip1 (human)

Site Information
GRkRRQtsMTDFyHs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447836

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 , 7 ) , flow cytometry ( 3 ) , mutation of modification site ( 3 , 4 , 5 , 6 ) , phospho-antibody ( 3 , 5 , 7 ) , phosphopeptide mapping ( 7 ) , western blotting ( 3 , 5 )
Disease tissue studied:
colorectal cancer ( 3 ) , colorectal carcinoma ( 3 ) , lung cancer ( 5 ) , non-small cell lung cancer ( 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 4 ) , 293E (epithelial) ( 6 ) , 3T3 (fibroblast) ( 4 , 5 ) , A172 (glial) ( 6 ) , COS (fibroblast) ( 5 ) , HCT116 (intestinal) ( 3 ) , NCI-H1299 (pulmonary) ( 5 ) , SF9 [p21Cip1 (human)] ( 7 ) , T98G (glial) ( 6 ) , U-118MG (glial) ( 6 ) , U87MG (glial) ( 6 )

Upstream Regulation
Regulatory protein:
Pim1 (human) ( 5 )
Putative in vivo kinases:
Akt1 (human) ( 6 ) , LATS2 (human) ( 2 )
Kinases, in vitro:
Akt1 (human) ( 6 ) , Pim1 (human) ( 5 ) , Pim2 (human) ( 3 ) , PKCA (human) ( 7 )
Treatments:
LY294002 ( 5 , 6 ) , okadaic_acid ( 7 ) , serum ( 6 ) , taxol ( 6 ) , wortmannin ( 5 )

Downstream Regulation
Effects of modification on p21Cip1:
intracellular localization ( 5 ) , molecular association, regulation ( 7 ) , protein stabilization ( 6 )
Effects of modification on biological processes:
cell growth, altered ( 5 )

References 

1

Esteve-Puig R, et al. (2014) A Mouse Model Uncovers LKB1 as an UVB-Induced DNA Damage Sensor Mediating CDKN1A (p21WAF1/CIP1) Degradation. PLoS Genet 10, e1004721
25329316   Curated Info

2

Suzuki H, et al. (2013) Lats2 phosphorylates p21/CDKN1A after UV irradiation and regulates apoptosis. J Cell Sci 126, 4358-68
23886938   Curated Info

3

Wang Z, et al. (2010) Pim-2 phosphorylation of p21(Cip1/WAF1) enhances its stability and inhibits cell proliferation in HCT116 cells. Int J Biochem Cell Biol 42, 1030-8
20307683   Curated Info

4

Densham RM, et al. (2009) MST kinases monitor actin cytoskeletal integrity and signal via c-Jun N-terminal kinase stress-activated kinase to regulate p21Waf1/Cip1 stability. Mol Cell Biol 29, 6380-90
19822666   Curated Info

5

Zhang Y, Wang Z, Magnuson NS (2007) Pim-1 kinase-dependent phosphorylation of p21Cip1/WAF1 regulates its stability and cellular localization in H1299 cells. Mol Cancer Res 5, 909-22
17855660   Curated Info

6

Li Y, Dowbenko D, Lasky LA (2002) AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival. J Biol Chem 277, 11352-61
11756412   Curated Info

7

Scott MT, Morrice N, Ball KL (2000) Reversible phosphorylation at the C-terminal regulatory domain of p21(Waf1/Cip1) modulates proliferating cell nuclear antigen binding. J Biol Chem 275, 11529-37
10753973   Curated Info