Ser37
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Home > Phosphorylation Site Page: > Ser37  -  TTK (human)

Site Information
EDLtDELsLNKISAD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2846500

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 , 7 ) , mass spectrometry ( 2 , 5 , 8 , 9 , 10 , 12 , 13 ) , mass spectrometry (in vitro) ( 4 , 11 ) , mutation of modification site ( 6 , 7 , 11 ) , phospho-antibody ( 1 , 3 ) , western blotting ( 3 )
Disease tissue studied:
luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 ) , cervical cancer ( 10 ) , cervical adenocarcinoma ( 10 ) , colorectal cancer ( 3 , 7 , 11 ) , colorectal carcinoma ( 3 , 7 , 11 )
Relevant cell line - cell type - tissue:
breast ( 2 ) , HCT116 (intestinal) ( 3 ) , HeLa (cervical) ( 1 , 5 , 6 , 8 , 13 ) , HeLa S3 (cervical) ( 10 , 12 ) , HeLa_Meta (cervical) ( 9 ) , HeLa_Pro (cervical) ( 9 ) , HeLa_Telo (cervical) ( 9 ) , SW480 (intestinal) ( 7 , 11 )

Upstream Regulation
Putative in vivo kinases:
TTK (human) ( 6 )
Kinases, in vitro:
PLK1 (human) ( 4 ) , TTK (human) ( 11 , 13 )
Putative upstream phosphatases:
PPP2R5D (human) ( 1 )
Treatments:
AZ3146 ( 3 ) , BI2536 ( 8 ) , CCT251455 ( 3 ) , MG132_withdrawal ( 9 ) , MLN8054 ( 8 ) , nocodazole ( 12 ) , siRNA ( 1 ) , thymidine ( 12 )

Downstream Regulation
Effects of modification on TTK:
enzymatic activity, induced ( 4 ) , intracellular localization ( 4 , 6 )
Effects of modification on biological processes:
cell cycle regulation ( 6 )

References 

1

Hayward D, et al. (2019) Checkpoint signaling and error correction require regulation of the MPS1 T-loop by PP2A-B56. J Cell Biol 218, 3188-3199
31511308   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Gurden MD, et al. (2015) Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance. Cancer Res 75, 3340-54
26202014   Curated Info

4

von Schubert C, et al. (2015) Plk1 and Mps1 Cooperatively Regulate the Spindle Assembly Checkpoint in Human Cells. Cell Rep 12, 66-78
26119734   Curated Info

5

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

6

Wang X, et al. (2014) Dynamic autophosphorylation of mps1 kinase is required for faithful mitotic progression. PLoS One 9, e104723
25265012   Curated Info

7

Zhang X, et al. (2011) Two LXXLL motifs in the N terminus of Mps1 are required for Mps1 nuclear import during G(2)/M transition and sustained spindle checkpoint responses. Cell Cycle 10, 2742-50
21778823   Curated Info

8

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

9

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

10

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

11

Xu Q, et al. (2009) Regulation of kinetochore recruitment of two essential mitotic spindle checkpoint proteins by Mps1 phosphorylation. Mol Biol Cell 20, 10-20
18923149   Curated Info

12

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

13

Jelluma N, et al. (2008) Chromosomal instability by inefficient Mps1 auto-activation due to a weakened mitotic checkpoint and lagging chromosomes. PLoS One 3, e2415
18545697   Curated Info