Ser59
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Home > Phosphorylation Site Page: > Ser59  -  Lck (human)

Site Information
EGSNPPAsPLQDNLV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447820

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 14 ) , electrophoretic mobility shift ( 14 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ) , mutation of modification site ( 1 , 11 , 12 , 14 ) , peptide sequencing ( 13 ) , phospho-antibody ( 1 , 2 ) , phosphoamino acid analysis ( 14 ) , phosphopeptide mapping ( 13 , 14 ) , western blotting ( 1 , 2 , 12 )
Disease tissue studied:
leukemia ( 1 ) , T cell leukemia ( 1 )
Relevant cell line - cell type - tissue:
171.CD4 ( 13 ) , HeLa (cervical) [CD4 (human)] ( 12 ) , HeLa (cervical) ( 11 , 14 ) , Jurkat (T lymphocyte) ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 12 , 13 ) , LSTRA (T lymphocyte) ( 13 ) , T lymphocyte-blood ( 2 )

Upstream Regulation
Regulatory protein:
PPP3CA (human) ( 1 )
Kinases, in vitro:
ERK1 (human) ( 13 , 14 ) , ERK2 (human) ( 14 )
Treatments:
anti-CD3 ( 13 ) , ciclosporin ( 1 ) , FK506 ( 1 ) , phorbol_ester ( 13 , 14 )

Downstream Regulation
Effects of modification on Lck:
enzymatic activity, inhibited ( 1 ) , molecular association, regulation ( 11 , 12 )
Effects of modification on biological processes:
cell adhesion, inhibited ( 1 )
Induce interaction with:
SQSTM1 (human) ( 11 )

References 

1

Dutta D, et al. (2017) Recruitment of calcineurin to the TCR positively regulates T cell activation. Nat Immunol 18, 196-204
27941787   Curated Info

2

Katz G, et al. (2014) SAP Facilitates Recruitment and Activation of LCK at NTB-A Receptors during Restimulation-Induced Cell Death. J Immunol 192, 4202-9
24688028   Curated Info

3

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

4

Guo A (2012) CST Curation Set: 13866; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

5

Mulhern D (2012) CST Curation Set: 13759; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

6

Possemato A (2010) CST Curation Set: 10530; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

7

Possemato A (2010) CST Curation Set: 10117; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

8

Possemato A (2009) CST Curation Set: 7997; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Possemato A (2009) CST Curation Set: 7999; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Possemato A (2007) CST Curation Set: 3482; Year: 2007; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Park I, et al. (1995) Phosphotyrosine-independent binding of a 62-kDa protein to the src homology 2 (SH2) domain of p56lck and its regulation by phosphorylation of Ser-59 in the lck unique N-terminal region. Proc Natl Acad Sci U S A 92, 12338-42
8618896   Curated Info

12

Joung I, et al. (1995) Modification of Ser59 in the unique N-terminal region of tyrosine kinase p56lck regulates specificity of its Src homology 2 domain. Proc Natl Acad Sci U S A 92, 5778-82
7597029   Curated Info

13

Watts JD, Sanghera JS, Pelech SL, Aebersold R (1993) Phosphorylation of serine 59 of p56lck in activated T cells. J Biol Chem 268, 23275-82
8226850   Curated Info

14

Winkler DG, et al. (1993) Phosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lck. Proc Natl Acad Sci U S A 90, 5176-80
8506364   Curated Info