Tyr265
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Home > Phosphorylation Site Page: > Tyr265  -  GJA1 (human)

Site Information
kDCGsQkyAyFNGCs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447815
Available spectra:  1 CST
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 4 , 5 , 7 , 8 , 9 , 10 , 12 , 13 , 14 , 15 , 16 , 17 ) , mutation of modification site ( 18 ) , phospho-antibody ( 11 , 18 ) , western blotting ( 11 , 18 )
Disease tissue studied:
breast cancer ( 4 ) , esophageal cancer ( 17 ) , leukemia ( 16 ) , acute myelogenous leukemia ( 16 ) , lung cancer ( 5 , 7 , 8 ) , non-small cell lung cancer ( 5 , 7 , 8 ) , non-small cell lung adenocarcinoma ( 5 , 8 ) , non-small cell large cell lung carcinoma ( 5 ) , ovarian cancer ( 14 , 15 ) , ovarian epithelial carcinoma ( 15 ) , multiple myeloma ( 12 , 13 ) , melanoma skin cancer ( 10 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 18 ) , HCC78 (pulmonary) ( 5 ) , heart ( 11 ) , HES-3 ('stem, embryonic') ( 9 ) , HMLER ('stem, breast cancer') ( 4 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 4 ) , KMS-11 (B lymphocyte) ( 12 , 13 ) , Kyse450 (squamous) ( 17 ) , lung ( 5 , 8 ) , ME-F2 (myeloid) ( 16 ) , myocyte-heart ( 18 ) , NCI-H1781 (pulmonary) ( 5 ) , NCI-H2228 (pulmonary) ( 5 ) , NCI-H3122 (pulmonary) ( 5 ) , NCI-H3255 (pulmonary) ( 7 ) , NCI-H661 (pulmonary) ( 5 ) , PA-1 (ovarian) ( 14 ) , skin ( 10 ) , TOV112D (ovarian) ( 15 )

Upstream Regulation
Putative in vivo kinases:
Src (rat) ( 18 )
Kinases, in vitro:
Src (human) ( 18 )

Downstream Regulation
Effects of modification on GJA1:
molecular association, regulation ( 18 ) , protein processing ( 18 )
Induce interaction with:
Src (human) ( 18 ) , ZO1 (human) ( 18 )

References 

1

Zheng L, et al. (2018) Phosphorylation of Cx43 residue Y313 by Src contributes to blocking the interaction with Drebrin and disassembling gap junctions. J Mol Cell Cardiol 126, 36-49
30448479   Curated Info

2

Yang X, et al. (2018) Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells. Radiat Res
30095367   Curated Info

3

Boeldt DS, et al. (2015) Phosphorylation of Ser-279/282 and Tyr-265 positions on Cx43 as possible mediators of VEGF-165 inhibition of pregnancy-adapted Ca2+ burst function in ovine uterine artery endothelial cells. Mol Cell Endocrinol 412, 73-84
26033246   Curated Info

4

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

5

Rikova K, Hall B (2013) CST Curation Set: 20740, 21167, 30170, 30171, 30172; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

6

Grosely R, et al. (2013) Effects of Phosphorylation on the Structure and Backbone Dynamics of the Intrinsically Disordered Connexin43 C-terminal Domain. J Biol Chem 288, 24857-70
23828237   Curated Info

7

Rikova K (2012) CST Curation Set: 13325; Year: 2012; Biosample/Treatment: cell line, NCI-H3255/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Ren H (2011) CST Curation Set: 11786; Year: 2011; Biosample/Treatment: cell line, RP103/untreated; Disease: non-small cell lung adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Ding VM, et al. (2011) Tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells. PLoS One 6, e17538
21437283   Curated Info

10

Tucker M (2009) CST Curation Set: 6391; Year: 2009; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Sato T, et al. (2008) Altered expression of connexin43 contributes to the arrhythmogenic substrate during the development of heart failure in cardiomyopathic hamster. Am J Physiol Heart Circ Physiol 294, H1164-73
18065522   Curated Info

12

Gu T (2006) CST Curation Set: 1727; Year: 2006; Biosample/Treatment: cell line, KMS-11/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Gu T (2006) CST Curation Set: 1728; Year: 2006; Biosample/Treatment: cell line, KMS-11/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

Guo A (2006) CST Curation Set: 1547; Year: 2006; Biosample/Treatment: cell line, PA-1/serum starved; Disease: ovarian cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

Guo A (2006) CST Curation Set: 1513; Year: 2006; Biosample/Treatment: cell line, TOV112D/serum starved; Disease: ovarian cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

16

Gu T (2006) CST Curation Set: 1399; Year: 2006; Biosample/Treatment: cell line, ME-F2/-; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

17

Yu J (2006) CST Curation Set: 1394; Year: 2006; Biosample/Treatment: cell line, Kyse450/-; Disease: esophageal cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

18

Toyofuku T, et al. (2001) c-Src regulates the interaction between connexin-43 and ZO-1 in cardiac myocytes. J Biol Chem 276, 1780-8
11035005   Curated Info