Ser529
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Home > Phosphorylation Site Page: > Ser529  -  NFkB-p65 (human)

Site Information
GLPNGLLsGDEDFSs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447799

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 5 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 3 , 6 ) , mutation of modification site ( 3 , 12 , 15 , 16 ) , phospho-antibody ( 3 , 4 , 8 , 9 , 10 , 11 , 13 , 14 , 16 ) , western blotting ( 3 , 4 , 8 , 9 , 10 , 16 )
Disease tissue studied:
gastric cancer ( 6 ) , gastric carcinoma ( 6 ) , lymphoma ( 4 , 9 ) , B cell lymphoma ( 4 ) , non-Hodgkin's lymphoma ( 4 ) , follicular lymphoma ( 4 ) , prostate cancer ( 8 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
CDK5RAP3 (human) ( 10 )
Putative in vivo kinases:
CK2A1 (human) ( 16 )
Kinases, in vitro:
CK2A1 (human) ( 16 )
Treatments:
2-deoxyglucose ( 7 ) , antitrypsin ( 9 ) , ciclosporin ( 5 ) , delphinidin ( 8 ) , IL-1b ( 3 ) , ionomycin ( 5 ) , LPS ( 9 ) , MG132 ( 16 ) , PD144795 ( 16 ) , phorbol_ester ( 5 ) , piceatannol ( 14 ) , silmitasertib ( 4 ) , TNF ( 10 , 14 , 16 )

Downstream Regulation
Effects of modification on NFkB-p65:
activity, induced ( 14 ) , intracellular localization ( 14 )
Effects of modification on biological processes:
transcription, altered ( 15 ) , transcription, induced ( 3 , 12 )

References 

1

Peltier D, et al. (2021) RNA-seq of human T cells after hematopoietic stem cell transplantation identifies as a regulator of T cell alloimmunity. Sci Transl Med 13
33731431   Curated Info

2

Gerner MC, et al. (2021) Packed red blood cells inhibit T-cell activation via ROS-dependent signaling pathways. J Biol Chem, 100487
33676898   Curated Info

3

Wang B, et al. (2015) Role of Novel Serine 316 Phosphorylation of the p65 Subunit of NF-κB in Differential Gene Regulation. J Biol Chem 290, 20336-47
26082493   Curated Info

4

Pizzi M, et al. (2015) Protein kinase CK2 is widely expressed in follicular, Burkitt and diffuse large B-cell lymphomas and propels malignant B-cell growth. Oncotarget 6, 6544-52
25788269   Curated Info

5

Frischbutter S, et al. (2011) Dephosphorylation of Bcl-10 by calcineurin is essential for canonical NF-κB activation in Th cells. Eur J Immunol 41, 2349-57
21674474   Curated Info

6

Moritz A (2010) CST Curation Set: 9234; Year: 2010; Biosample/Treatment: cell line, MKN-45/calyculin_A & pervanadate; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

7

Zhong D, et al. (2009) The Glycolytic Inhibitor 2-Deoxyglucose Activates Multiple Prosurvival Pathways through IGF1R. J Biol Chem 284, 23225-33
19574224   Curated Info

8

Hafeez BB, et al. (2008) A dietary anthocyanidin delphinidin induces apoptosis of human prostate cancer PC3 cells in vitro and in vivo: involvement of nuclear factor-kappaB signaling. Cancer Res 68, 8564-72
18922932   Curated Info

9

Bristow CL, et al. (2008) NF-kappaB signaling, elastase localization, and phagocytosis differ in HIV-1 permissive and nonpermissive U937 clones. J Immunol 180, 492-9
18097051   Curated Info

10

Wang J, et al. (2007) LZAP, a putative tumor suppressor, selectively inhibits NF-kappaB. Cancer Cell 12, 239-51
17785205   Curated Info

11

Xu C, et al. (2005) Suppression of NF-kappaB and NF-kappaB-regulated gene expression by sulforaphane and PEITC through IkappaBalpha, IKK pathway in human prostate cancer PC-3 cells. Oncogene 24, 4486-95
15856023   Curated Info

12

O'Mahony AM, et al. (2004) Human T-cell lymphotropic virus type 1 tax induction of biologically Active NF-kappaB requires IkappaB kinase-1-mediated phosphorylation of RelA/p65. J Biol Chem 279, 18137-45
14963024   Curated Info

13

McNulty SE, et al. (2004) Comparative expression of NFkappaB proteins in melanocytes of normal skin vs. benign intradermal naevus and human metastatic melanoma biopsies. Pigment Cell Res 17, 173-80
15016307   Curated Info

14

Ashikawa K, et al. (2002) Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol 169, 6490-7
12444159   Curated Info

15

Madrid LV, Mayo MW, Reuther JY, Baldwin AS (2001) Akt stimulates the transactivation potential of the RelA/p65 Subunit of NF-kappa B through utilization of the Ikappa B kinase and activation of the mitogen-activated protein kinase p38. J Biol Chem 276, 18934-40
11259436   Curated Info

16

Wang D, Westerheide SD, Hanson JL, Baldwin AS (2000) Tumor necrosis factor alpha-induced phosphorylation of RelA/p65 on Ser529 is controlled by casein kinase II. J Biol Chem 275, 32592-7
10938077   Curated Info