Ser145
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Home > Phosphorylation Site Page: > Ser145  -  Mad1 (human)

Site Information
IERIRMDsIGSTVSS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2807903

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 2 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 , 2 ) , western blotting ( 1 , 2 )
Disease tissue studied:
breast cancer ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
Akt1 (human) ( 2 ) , p70S6K (human) ( 2 ) , p90RSK (human) ( 2 )
Putative in vivo kinases:
Akt1 (human) ( 1 ) , p70S6K (human) ( 1 , 2 ) , p90RSK (human) ( 2 )
Kinases, in vitro:
Akt1 (human) ( 1 , 2 ) , p70S6K (human) ( 2 ) , p90RSK (human) ( 2 )
Treatments:
insulin ( 2 ) , LY294002 ( 2 ) , PD98059 ( 1 , 2 ) , rapamycin ( 1 , 2 ) , serum ( 2 ) , siRNA ( 2 )

Downstream Regulation
Effects of modification on Mad1:
molecular association, regulation ( 1 ) , protein degradation ( 2 )
Effects of modification on biological processes:
cell cycle regulation ( 1 ) , cell growth, altered ( 2 ) , transcription, induced ( 1 , 2 )
Induce interaction with:
DNA ( 1 )

References 

1

Chou CK, et al. (2009) The suppression of MAD1 by AKT-mediated phosphorylation activates MAD1 target genes transcription. Mol Carcinog 48, 1048-58
19526459   Curated Info

2

Zhu J, Blenis J, Yuan J (2008) Activation of PI3K/Akt and MAPK pathways regulates Myc-mediated transcription by phosphorylating and promoting the degradation of Mad1. Proc Natl Acad Sci U S A 105, 6584-9
18451027   Curated Info