Ser20
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Home > Phosphorylation Site Page: > Ser20  -  CHIP (rat)

Site Information
LGtGGGGsPDKsPSA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 453857

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 'brain, cerebral cortex' ( 3 ) , 'kidney, cortex' ( 4 ) , 293 (epithelial) ( 1 ) , adipose tissue ( 3 ) , heart ( 3 ) , IEC-6 (epithelial) ( 2 ) , kidney ( 3 ) , liver ( 3 , 5 ) , lung ( 3 ) , myocyte-heart ( 1 ) , pancreas ( 3 ) , spleen ( 3 ) , testis ( 3 ) , thymus ( 3 )

Upstream Regulation
Putative in vivo kinases:
PKG1 (rat) ( 1 )
Kinases, in vitro:
PKG1 (rat) ( 1 )
Treatments:
cGMP_analog ( 1 ) , DT-3 ( 1 ) , serum ( 2 ) , sildenafil ( 1 )

Downstream Regulation
Effects of modification on CHIP:
molecular association, regulation ( 1 ) , protein stabilization ( 1 )
Induce interaction with:
HSC70 (rat) ( 1 )

References 

1

Ranek MJ, et al. (2020) CHIP phosphorylation by protein kinase G enhances protein quality control and attenuates cardiac ischemic injury. Nat Commun 11, 5237
33082318   Curated Info

2

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

3

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

4

Feric M, et al. (2011) Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubule. Am J Physiol Cell Physiol 300, C755-70
21209370   Curated Info

5

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info