Tyr693
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Home > Phosphorylation Site Page: > Tyr693  -  STAT4 (human)

Site Information
TERGDkGyVPSVFIP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447790
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 1 ) , immunoassay ( 2 ) , immunoprecipitation ( 1 , 2 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 8 , 9 , 10 ) , modification-specific antibody ( 1 ) , mutation of modification site ( 1 ) , phospho-antibody ( 2 , 6 , 7 , 11 ) , western blotting ( 1 , 2 , 6 , 7 , 11 )
Disease tissue studied:
HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 ) , leukemia ( 4 , 5 , 8 , 10 ) , acute lymphocytic leukemia ( 8 , 10 ) , acute myelogenous leukemia ( 5 ) , chronic lymphocytic leukemia ( 4 ) , lymphoma ( 9 ) , Hodgkin's lymphoma ( 9 )
Relevant cell line - cell type - tissue:
2D6 (T lymphocyte) ( 6 ) , 3T3 (fibroblast) ( 1 ) , breast ( 3 ) , CD4+ (T lymphocyte) ( 2 ) , F15R-Kit (T lymphocyte) ( 4 ) , HEK293T (epithelial) ( 1 , 2 , 6 ) , IEC-6 (epithelial) ( 2 ) , KG-1 (myeloid) ( 5 ) , L428 (lymphoid) ( 9 ) , lymphocyte ( 1 ) , mononuclear ( 11 ) , spleen ( 2 ) , SUP-T13 (T lymphocyte) ( 8 , 10 ) , T lymphocyte-blood ( 7 )

Upstream Regulation
Regulatory protein:
Tyk2 (human) ( 7 )
Putative upstream phosphatases:
PTPN13 (human) ( 6 )
Treatments:
ciclosporin ( 11 ) , dextran sulphate sodium ( 2 ) , gandotinib ( 2 ) , IFN-alpha ( 6 , 7 ) , IL-12 ( 7 , 11 ) , IL-15 ( 4 ) , IL-2 ( 4 ) , IL-23 ( 7 ) , LIF ( 2 ) , rapamycin ( 11 )

Downstream Regulation
Effects of modification on STAT4:
intracellular localization ( 2 ) , molecular association, regulation ( 2 )
Effects of modification on biological processes:
cell differentiation, inhibited ( 2 )
Induce interaction with:
LIFR (human) ( 2 ) , STAT3 (human) ( 2 ) , STAT4 (human) ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
Crohn disease ( 2 )

References 

1

Zhang YS, et al. (2022) Acetylation licenses Th1 cell polarization to constrain Listeria monocytogenes infection. Cell Death Differ 29
35614130   Curated Info

2

Zhang YS, et al. (2019) STAT4 activation by leukemia inhibitory factor confers a therapeutic effect on intestinal inflammation. EMBO J 38
30770344   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Arneja A, et al. (2014) Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration. J Immunol 192, 123-35
24298013   Curated Info

5

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

6

Nakahira M, et al. (2007) Regulation of signal transducer and activator of transcription signaling by the tyrosine phosphatase PTP-BL. Immunity 26, 163-76
17306571   Curated Info

7

Minegishi Y, et al. (2006) Human tyrosine kinase 2 deficiency reveals its requisite roles in multiple cytokine signals involved in innate and acquired immunity. Immunity 25, 745-55
17088085   Curated Info

8

Gu T (2006) CST Curation Set: 1790; Year: 2006; Biosample/Treatment: cell line, SUP-T13/untreated; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Gu T (2006) CST Curation Set: 1626; Year: 2006; Biosample/Treatment: cell line, L428/untreated; Disease: Hodgkin's lymphoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Gu T (2005) CST Curation Set: 731; Year: 2005; Biosample/Treatment: cell line, SUP-T13/-; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Kusaba H, et al. (2005) Interleukin-12-induced interferon-gamma production by human peripheral blood T cells is regulated by mammalian target of rapamycin (mTOR). J Biol Chem 280, 1037-43
15522880   Curated Info