Lys194
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.0.1
Powered by Cell Signaling Technology
Home > Sumoylation Site Page: > Lys194  -  STF-1 (mouse)

Site Information
AFSNRTIkSEYPEPY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2798300

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 1 , 2 , 5 ) , immunoprecipitation ( 3 , 4 , 5 ) , modification-specific antibody ( 5 ) , mutation of modification site ( 1 , 2 , 3 , 4 , 5 , 6 ) , western blotting ( 2 , 5 )
Disease tissue studied:
adrenal cancer ( 1 , 3 , 5 ) , adrenocortical carcinoma ( 1 , 3 , 5 ) , breast cancer ( 1 ) , gestational cancer ( 1 ) , gestational trophoblastic choriocarcinoma ( 1 ) , liver cancer ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 , 4 , 6 ) , alphaT3-1 (pituitary cell) ( 5 ) , COS (fibroblast) ( 3 ) , COS7 (fibroblast) ( 5 ) , endocrine ( 2 ) , HeLa (cervical) ( 5 ) , HepG2 (hepatic) ( 1 ) , JEG-3 (uterine) ( 1 ) , MCF-7 (breast cell) ( 1 ) , Y-1 (adrenal cortical) ( 1 , 3 , 5 )

Upstream Regulation
Regulatory protein:
GEMIN3 (human) ( 5 ) , PIAS1 (mouse) ( 5 ) , PIAS3 (mouse) ( 5 )
Treatments:
ACTH ( 3 )

Downstream Regulation
Effects of modification on STF-1:
activity, inhibited ( 3 ) , molecular association, regulation ( 1 , 5 ) , phosphorylation ( 3 )
Effects of modification on biological processes:
signaling pathway regulation ( 2 ) , transcription, altered ( 2 , 3 ) , transcription, inhibited ( 1 , 5 , 6 )
Induce interaction with:
GEMIN3 (human) ( 5 )
Inhibit interaction with:
CDK7 (human) ( 1 ) , SRC-1 (human) ( 1 )

References 

1

Wang CM, Liu R, Wang L, Yang WH (2013) Acidic residue Glu199 increases SUMOylation level of nuclear hormone receptor NR5A1. Int J Mol Sci 14, 22331-45
24232453   Curated Info

2

Lee FY, et al. (2011) Eliminating SF-1 (NR5A1) sumoylation in vivo results in ectopic hedgehog signaling and disruption of endocrine development. Dev Cell 21, 315-27
21820362   Curated Info

3

Yang WH, et al. (2009) SUMOylation inhibits SF-1 activity by reducing CDK7-mediated serine 203 phosphorylation. Mol Cell Biol 29, 613-25
19015234   Curated Info

4

Campbell LA, et al. (2008) Decreased recognition of SUMO-sensitive target genes following modification of SF-1 (NR5A1). Mol Cell Biol 28, 7476-86
18838537   Curated Info

5

Lee MB, et al. (2005) The DEAD-box protein DP103 (Ddx20 or Gemin-3) represses orphan nuclear receptor activity via SUMO modification. Mol Cell Biol 25, 1879-90
15713642   Curated Info

6

Komatsu T, et al. (2004) Small ubiquitin-like modifier 1 (SUMO-1) modification of the synergy control motif of Ad4 binding protein/steroidogenic factor 1 (Ad4BP/SF-1) regulates synergistic transcription between Ad4BP/SF-1 and Sox9. Mol Endocrinol 18, 2451-62
15192080   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2019 Cell Signaling Technology, Inc.