Lys153
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Home > Sumoylation Site Page: > Lys153  -  TOP1 (human)

Site Information
DykPKKIkTEDTkKE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2793909

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 3 ) , immunoassay ( 5 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 3 , 4 , 5 ) , western blotting ( 3 , 4 , 5 )
Disease tissue studied:
bone cancer ( 3 ) , colorectal carcinoma ( 5 ) , fibrosarcoma of soft tissue ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
UBE2H (human) ( 4 )
Treatments:
camptothecin ( 4 ) , CPT ( 3 )

Downstream Regulation
Effects of modification on TOP1:
intracellular localization ( 5 ) , molecular association, regulation ( 4 )
Effects of modification on biological processes:
apoptosis, induced ( 4 )
Induce interaction with:
DNA ( 4 )

References 

1

Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36
25218447   Curated Info

2

Tammsalu T, et al. (2014) Proteome-wide identification of SUMO2 modification sites. Sci Signal 7, rs2
24782567   Curated Info

3

Hammer E, Heilbronn R, Weger S (2007) The E3 ligase Topors induces the accumulation of polysumoylated forms of DNA topoisomerase I in vitro and in vivo. FEBS Lett 581, 5418-24
17976381   Curated Info

4

Horie K, et al. (2002) SUMO-1 conjugation to intact DNA topoisomerase I amplifies cleavable complex formation induced by camptothecin. Oncogene 21, 7913-22
12439742   Curated Info

5

Rallabhandi P, et al. (2002) Sumoylation of topoisomerase I is involved in its partitioning between nucleoli and nucleoplasm and its clearing from nucleoli in response to camptothecin. J Biol Chem 277, 40020-6
12149243   Curated Info