Lys117
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Home > Sumoylation Site Page: > Lys117  -  TOP1 (human)

Site Information
FssPPQIkDEPEDDG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2793907

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 6 ) , immunoassay ( 9 ) , immunoprecipitation ( 8 ) , mass spectrometry ( 2 , 4 , 5 ) , mutation of modification site ( 6 , 7 , 8 , 9 ) , western blotting ( 6 , 8 , 9 )
Disease tissue studied:
bone cancer ( 6 ) , colorectal carcinoma ( 9 ) , fibrosarcoma of soft tissue ( 8 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
UBE2H (human) ( 8 )
Treatments:
camptothecin ( 8 ) , CPT ( 6 )

Downstream Regulation
Effects of modification on TOP1:
intracellular localization ( 9 ) , molecular association, regulation ( 8 )
Effects of modification on biological processes:
apoptosis, induced ( 8 )
Induce interaction with:
DNA ( 8 )

References 

1

Lumpkin RJ, et al. (2017) Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. Nat Commun 8, 1171
29079793   Curated Info

2

Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36
25218447   Curated Info

3

Impens F, Radoshevich L, Cossart P, Ribet D (2014) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli. Proc Natl Acad Sci U S A 111, 12432-7
25114211   Curated Info

4

Tammsalu T, et al. (2014) Proteome-wide identification of SUMO2 modification sites. Sci Signal 7, rs2
24782567   Curated Info

5

Matic I, et al. (2010) Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif. Mol Cell 39, 641-52
20797634   Curated Info

6

Hammer E, Heilbronn R, Weger S (2007) The E3 ligase Topors induces the accumulation of polysumoylated forms of DNA topoisomerase I in vitro and in vivo. FEBS Lett 581, 5418-24
17976381   Curated Info

7

Yang M, et al. (2006) Assembly of a polymeric chain of SUMO1 on human topoisomerase I in vitro. J Biol Chem 281, 8264-74
16428803   Curated Info

8

Horie K, et al. (2002) SUMO-1 conjugation to intact DNA topoisomerase I amplifies cleavable complex formation induced by camptothecin. Oncogene 21, 7913-22
12439742   Curated Info

9

Rallabhandi P, et al. (2002) Sumoylation of topoisomerase I is involved in its partitioning between nucleoli and nucleoplasm and its clearing from nucleoli in response to camptothecin. J Biol Chem 277, 40020-6
12149243   Curated Info