Thr231
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Home > Phosphorylation Site Page: > Thr231  -  RIPK3 (mouse)

Site Information
EAELVDKtsLIRETV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 27928506

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 11 ) , immunoassay ( 3 , 4 , 6 ) , immunoprecipitation ( 6 , 7 , 11 ) , mass spectrometry ( 6 , 11 ) , mutation of modification site ( 8 , 9 , 10 , 11 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 10 , 11 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 )
Disease tissue studied:
colorectal cancer ( 1 , 5 ) , colorectal carcinoma ( 1 , 5 ) , lymphoma ( 5 ) , fibrosarcoma of soft tissue ( 5 , 8 , 10 , 11 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 3 ) , 3T3 (fibroblast) ( 5 , 9 , 11 ) , E.coli (bacterial) ( 11 ) , embryo ( 4 ) , HEK293T (epithelial) ( 1 , 10 , 11 ) , HT-29 (intestinal) ( 1 , 5 ) , L929 (fibroblast) ( 5 , 8 , 10 , 11 ) , macrophage-bone marrow ( 6 ) , MEF (fibroblast) ( 1 , 2 , 4 , 6 , 7 , 10 ) , neuron-brain ( 3 ) , U-937 (myeloid) ( 5 )

Upstream Regulation
Regulatory protein:
CASP8 (human) ( 4 ) , CK1G2 (mouse) ( 1 ) , FAM105B (human) ( 6 ) , RIPK1 (human) ( 4 ) , RIPK1 (mouse) ( 3 ) , RIPK3 (mouse) ( 3 ) , ULK1 (human) ( 2 )
Putative in vivo kinases:
RIPK3 (human) ( 6 , 11 )
Kinases, in vitro:
RIPK3 (mouse) ( 11 )
Treatments:
cycloheximide ( 4 ) , ischemia/reperfusion ( 3 ) , LPS ( 1 ) , phenhydan ( 5 ) , TNF ( 1 , 2 , 4 , 5 , 6 , 7 , 11 ) , TRAIL ( 1 ) , Z-VAD-FMK ( 1 , 2 , 5 , 6 , 7 , 11 )

Downstream Regulation
Effects of modification on RIPK3:
intracellular localization ( 11 ) , molecular association, regulation ( 10 , 11 ) , phosphorylation ( 10 ) , protein conformation ( 10 )
Effects of modification on biological processes:
apoptosis, induced ( 4 , 9 , 10 ) , apoptosis, inhibited ( 11 )
Induce interaction with:
MLKL (mouse) ( 10 , 11 )

References 

1

Li D, et al. (2020) Casein kinase 1G2 suppresses necroptosis-promoted testis aging by inhibiting receptor-interacting kinase 3. Elife 9
33206046   Curated Info

2

Wu W, et al. (2020) The Autophagy-Initiating Kinase ULK1 Controls RIPK1-Mediated Cell Death. Cell Rep 31, 107547
32320653   Curated Info

3

Naito MG, et al. (2020) Sequential activation of necroptosis and apoptosis cooperates to mediate vascular and neural pathology in stroke. Proc Natl Acad Sci U S A
32071228   Curated Info

4

Newton K, et al. (2019) Cleavage of RIPK1 by caspase-8 is crucial for limiting apoptosis and necroptosis. Nature 574, 428-431
31511692   Curated Info

5

Moerke C, et al. (2018) The anticonvulsive Phenhydan suppresses extrinsic cell death. Cell Death Differ
30442947   Curated Info

6

Heger K, et al. (2018) OTULIN limits cell death and inflammation by deubiquitinating LUBAC. Nature 559, 120-124
29950720   Curated Info

7

Newton K, et al. (2016) RIPK1 inhibits ZBP1-driven necroptosis during development. Nature 540, 129-133
27819682   Curated Info

8

Chen W, et al. (2015) Ppm1b negatively regulates necroptosis through dephosphorylating Rip3. Nat Cell Biol 17, 434-44
25751141   Curated Info

9

Mandal P, et al. (2014) RIP3 induces apoptosis independent of pronecrotic kinase activity. Mol Cell 56, 481-95
25459880   Curated Info

10

Wu XN, et al. (2014) Distinct roles of RIP1-RIP3 hetero- and RIP3-RIP3 homo-interaction in mediating necroptosis. Cell Death Differ 21, 1709-20
24902902   Curated Info

11

Chen W, et al. (2013) Diverse sequence determinants control human and mouse receptor interacting protein 3 (RIP3) and mixed lineage kinase domain-like (MLKL) interaction in necroptotic signaling. J Biol Chem 288, 16247-61
23612963   Curated Info