Ser433
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Home > Phosphorylation Site Page: > Ser433  -  NRF2 (human)

Site Information
PEKELPVsPGHrktP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 27461383

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 , 5 ) , mass spectrometry ( 2 , 3 , 4 , 6 ) , mutation of modification site ( 1 , 5 ) , western blotting ( 2 , 5 )
Disease tissue studied:
HER2 positive breast cancer ( 4 ) , luminal A breast cancer ( 4 ) , luminal B breast cancer ( 4 ) , breast cancer, surrounding tissue ( 4 ) , breast cancer, triple negative ( 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 ) , astrocyte ( 5 ) , breast ( 4 ) , HeLa (cervical) ( 3 , 6 ) , MEF (fibroblast) ( 1 , 2 )

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (human) ( 1 , 2 )
Kinases, in vitro:
AMPKA1 (human) ( 2 ) , CDK5 (human) ( 5 )
Treatments:
A-769662 ( 1 , 2 ) , compound_C ( 2 ) , MG132 ( 2 )

Downstream Regulation
Effects of modification on NRF2:
activity, induced ( 2 ) , intracellular localization ( 5 ) , molecular association, regulation ( 1 , 2 ) , protein degradation ( 1 ) , protein stabilization ( 2 ) , ubiquitination ( 1 )
Effects of modification on biological processes:
signaling pathway regulation ( 2 ) , transcription, induced ( 2 ) , transcription, inhibited ( 1 )
Induce interaction with:
BTRC (human) ( 2 ) , FBW1B (human) ( 1 )

References 

1

Petsouki E, Ender S, Sosa Cabrera SN, Heiss EH (2023) AMPK-Mediated Phosphorylation of Nrf2 at S374/S408/S433 Favors Its ╬▓TrCP2-Mediated Degradation in KEAP1-Deficient Cells. Antioxidants (Basel) 12
37627580   Curated Info

2

Matzinger M, et al. (2020) AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes. Redox Biol 29, 101393
31805502   Curated Info

3

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

4

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

5

Jimenez-Blasco D, et al. (2015) Astrocyte NMDA receptors' activity sustains neuronal survival through a Cdk5-Nrf2 pathway. Cell Death Differ 22, 1877-89
25909891   Curated Info

6

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

7

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info