Thr108

Site Information
VrHHCPNtPIILVGT SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 27458396

In vivo Characterization
Methods used to characterize site in vivo:	[32P] ATP in vitro ( 4 ) , immunoprecipitation ( 3 ) , mutation of modification site ( 1 , 2 , 3 , 4 ) , western blotting ( 3 )
Disease tissue studied:	breast cancer ( 4 )
Relevant cell line - cell type - tissue:	COS7 (fibroblast) ( 1 , 3 , 4 ) , E.coli (bacterial) ( 2 ) , HEK293T (epithelial) ( 1 ) , MCF-7 (breast cell) ( 4 ) , MDA-MB-231 (breast cell) ( 4 )

Downstream Regulation
Effects of modification on RAC1:	enzymatic activity, inhibited ( 4 ) , intracellular localization ( 1 , 4 ) , molecular association, regulation ( 1 , 4 )
Effects of modification on biological processes:	RNA splicing, induced ( 1 )
Induce interaction with:	SFRS2 (human) ( 1 ) , hnRNP A1 (human) ( 1 ) , snRNP A' (human) ( 1 )
Inhibit interaction with:	PLCG1 (human) ( 4 )

References
1	Abdrabou A, Wang Z (2021) Regulation of the nuclear speckle localization and function of Rac1. FASEB J 35, e21235 33417283 Curated Info
2	Brandwein D, et al. (2018) An In Vitro Kinase Assay to Assess Rac1 Phosphorylation by ERK. Methods Mol Biol 1821, 131-140 30062409 Curated Info
3	Tong J, Li L, Ballermann B, Wang Z (2016) Phosphorylation and Activation of RhoA by ERK in Response to Epidermal Growth Factor Stimulation. PLoS One 11, e0147103 26816343 Curated Info
4	Tong J, Li L, Ballermann B, Wang Z (2013) Phosphorylation of Rac1 T108 by extracellular signal-regulated kinase in response to epidermal growth factor: a novel mechanism to regulate Rac1 function. Mol Cell Biol 33, 4538-51 24043306 Curated Info
5	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info