Ser434

Site Information
VEVCkDEsVDLEEFR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 27447299

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 4 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:	HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) [DGCR8 (human), transfection] ( 4 ) , breast ( 2 ) , HCT116 (intestinal) ( 1 )

Downstream Regulation
Effects of modification on DGCR8:	protein stabilization ( 4 )

References
1	Calses PC, et al. (2017) DGCR8 Mediates Repair of UV-Induced DNA Damage Independently of RNA Processing. Cell Rep 19, 162-174 28380355 Curated Info
2	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
3	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
4	Herbert KM, et al. (2013) Phosphorylation of DGCR8 increases its intracellular stability and induces a progrowth miRNA profile. Cell Rep 5, 1070-81 24239349 Curated Info
5	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info