Ser52
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Home > Phosphorylation Site Page: > Ser52  -  AMBRA1 (human)

Site Information
KRVELPDsPRSTFLL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 27443426

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 4 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 4 , 5 ) , mutation of modification site ( 1 , 4 ) , phospho-antibody ( 4 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
mTOR (human) ( 4 )
Kinases, in vitro:
mTOR (human) ( 4 )
Treatments:
starvation_medium ( 4 ) , Torin1 ( 4 )

Downstream Regulation
Effects of modification on AMBRA1:
activity, inhibited ( 4 ) , enzymatic activity, inhibited ( 4 ) , protein degradation ( 4 ) , ubiquitination ( 4 )
Effects of modification on biological processes:
autophagy, inhibited ( 4 ) , signaling pathway regulation ( 4 )

References 

1

Antonioli M, et al. (2014) AMBRA1 Interplay with Cullin E3 Ubiquitin Ligases Regulates Autophagy Dynamics. Dev Cell 31, 734-46
25499913   Curated Info

2

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

3

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

4

Nazio F, et al. (2013) mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6. Nat Cell Biol 15, 406-16
23524951   Curated Info

5

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info