Arg461
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Home > Mono-methylation Site Page: > Arg461  -  CstF-64T (human)

Site Information
DARGLEMrGPVPssR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 27277621
Available spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 )
Disease tissue studied:
lung cancer ( 5 , 6 , 7 , 8 , 9 ) , non-small cell lung cancer ( 7 , 8 , 9 , 10 , 11 ) , non-small cell lung adenocarcinoma ( 7 , 8 , 9 , 10 ) , non-small cell large cell lung carcinoma ( 7 , 9 ) , small-cell lung cancer ( 5 , 6 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 ) , 293T (epithelial) [PADI4 (human), no information, doxycyline inducible over-expression of PADI4] ( 3 ) , Calu-3 (pulmonary) ( 8 ) , DMS53 (pulmonary) ( 6 ) , DMS79 (pulmonary) ( 5 ) , HCC78 (pulmonary) ( 9 ) , HCC827 (pulmonary) ( 8 ) , HCT116 (intestinal) ( 4 , 12 ) , lung ( 5 , 6 , 7 , 8 , 9 ) , NCI-H128 (pulmonary) ( 5 ) , NCI-H1299 (pulmonary) ( 7 ) , NCI-H1417 (pulmonary) ( 5 ) , NCI-H1650 (pulmonary) ( 8 ) , NCI-H1734 (pulmonary) ( 7 ) , NCI-H1781 (pulmonary) ( 9 ) , NCI-H1944 (pulmonary) ( 7 ) , NCI-H1975 (pulmonary) ( 8 ) , NCI-H209 (pulmonary) ( 5 ) , NCI-H2106 (pulmonary) ( 8 ) , NCI-H2228 (pulmonary) ( 9 , 10 ) , NCI-H3122 (pulmonary) ( 9 ) , NCI-H3255 (pulmonary) ( 11 ) , NCI-H358 (pulmonary) ( 7 ) , NCI-H446 (pulmonary) ( 6 ) , NCI-H460 (pulmonary) ( 7 ) , NCI-H524 (pulmonary) ( 5 ) , NCI-H526 (pulmonary) ( 6 ) , NCI-H661 (pulmonary) ( 9 ) , NCI-H69 (pulmonary) ( 6 ) , NCI-H82 (pulmonary) ( 6 ) , T lymphocyte ( 2 ) , U2OS (bone cell) ( 3 )

References 

1

Larsen SC, et al. (2016) Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells. Sci Signal 9, rs9
27577262   Curated Info

2

Geoghegan V, et al. (2015) Comprehensive identification of arginine methylation in primary T cells reveals regulatory roles in cell signalling. Nat Commun 6, 6758
25849564   Curated Info

3

Sylvestersen KB, et al. (2014) Proteomic analysis of arginine methylation sites in human cells reveals dynamic regulation during transcriptional arrest. Mol Cell Proteomics 13, 2072-88
24563534   Curated Info

4

Guo A, et al. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation. Mol Cell Proteomics 13, 372-87
24129315   Curated Info

5

Rikova K (2013) CST Curation Set: 20094; Year: 2013; Biosample/Treatment: cell line, H1417, DMS79, H128, H209, H524; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

6

Rikova K (2013) CST Curation Set: 20095; Year: 2013; Biosample/Treatment: cell line, DMS53, H526, H69, H82, H446; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

7

Rikova K (2013) CST Curation Set: 20097; Year: 2013; Biosample/Treatment: cell line, H1299, H1944, H358, H1734, H460; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

8

Rikova K (2013) CST Curation Set: 20098; Year: 2013; Biosample/Treatment: cell line, H1650, HCC827, H1975, Calu-3, H2106; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

9

Rikova K (2013) CST Curation Set: 20100; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

10

Rikova K (2013) CST Curation Set: 18850; Year: 2013; Biosample/Treatment: cell line, H2228/crizotinib, geldanamycin; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

11

Rikova K (2012) CST Curation Set: 16035; Year: 2012; Biosample/Treatment: cell line, H3255/iressa; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

12

Mulhern D (2011) CST Curation Set: 13243; Year: 2011; Biosample/Treatment: cell line, HCT116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine (R*GG) (D5A12) Rabbit mAb Cat#: 8711, PTMScan(R) Mono-Methyl Arginine (D5A12) Immunoaffinity Beads Cat#: 8963
Curated Info