Thr129
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr129  -  PKM (human)

Site Information
GLIkGsGtAEVELkk   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 27110042

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 1 ) , mass spectrometry ( 2 , 3 , 4 , 6 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 1 )
Disease tissue studied:
breast cancer ( 3 ) , breast ductal carcinoma ( 3 ) , breast cancer, triple negative ( 3 ) , lung cancer ( 1 , 4 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 , 4 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 6 ) , 293 (epithelial) ( 1 ) , A549 (pulmonary) ( 1 ) , breast ( 3 ) , HeLa (cervical) ( 2 ) , lung ( 4 )

Upstream Regulation
Putative in vivo kinases:
EEF2K (human) ( 1 )
Kinases, in vitro:
EEF2K (human) ( 1 )
Treatments:
A484954 ( 1 ) , siRNA ( 1 )

Downstream Regulation
Effects of modification on PKM:
molecular association, regulation ( 1 ) , phosphorylation ( 1 )
Inhibit interaction with:
PKM (human) ( 1 )

References 

1

Xiao M, et al. (2020) The eEF2 kinase-induced STAT3 inactivation inhibits lung cancer cell proliferation by phosphorylation of PKM2. Cell Commun Signal 18, 25
32054489   Curated Info

2

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

5

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

6

Højlund K, et al. (2009) In vivo phosphoproteome of human skeletal muscle revealed by phosphopeptide enrichment and HPLC-ESI-MS/MS. J Proteome Res 8, 4954-65
19764811   Curated Info