Ser187
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.1
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser187  -  FEN1 (human)

Site Information
MDCLTFGsPVLMrHL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2708400

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 1 ) , mass spectrometry (in vitro) ( 2 ) , mutation of modification site ( 1 , 2 , 3 , 4 ) , phospho-antibody ( 4 ) , western blotting ( 1 , 2 , 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CDK1 (human) ( 4 )
Kinases, in vitro:
CDK1 (human) ( 4 )
Treatments:
olomoucine ( 4 ) , UV ( 3 )

Downstream Regulation
Effects of modification on FEN1:
enzymatic activity, inhibited ( 4 ) , intracellular localization ( 2 , 3 ) , molecular association, regulation ( 2 , 4 ) , protein degradation ( 1 ) , sumoylation ( 1 )
Effects of modification on biological processes:
cell cycle regulation ( 2 , 4 )
Inhibit interaction with:
PCNA (human) ( 2 , 4 )

References 

1

Guo Z, et al. (2012) Sequential Posttranslational Modifications Program FEN1 Degradation during Cell-Cycle Progression. Mol Cell 47, 444-56
22749529   Curated Info

2

Guo Z, et al. (2010) Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding. Nat Chem Biol 6, 766-73
20729856   Curated Info

3

Guo Z, et al. (2008) Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair. Mol Cell Biol 28, 4310-9
18443037   Curated Info

4

Henneke G, Koundrioukoff S, Hübscher U (2003) Phosphorylation of human Fen1 by cyclin-dependent kinase modulates its role in replication fork regulation. Oncogene 22, 4301-13
12853968   Curated Info