Ser550
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Home > Phosphorylation Site Page: > Ser550  -  NHE3 (mouse)

Site Information
AEGERRGsLAFIRSP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450899

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 8 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 7 ) , western blotting ( 1 , 2 , 3 , 4 , 6 , 7 )
Relevant cell line - cell type - tissue:
'kidney, tubule' ( 2 ) , gallbladder ( 3 ) , ileum ( 7 ) , kidney ( 1 , 4 , 6 , 8 )

Upstream Regulation
Regulatory protein:
NHERF-2 (mouse) ( 7 )
Treatments:
alogliptin ( 6 ) , caffeine ( 4 ) , empagliflozin ( 1 ) , excendin-4 ( 6 ) , GLP-1 ( 6 ) , hypertonic_buffer ( 2 ) , lithogenic diet ( 3 ) , low Na ( 2 ) , NKH_477 ( 7 )

References 

1

Onishi A, et al. (2020) A role for the tubular Na-H-exchanger NHE3 in the natriuretic effect of the SGLT2 inhibitor empagliflozin. Am J Physiol Renal Physiol
32893663   Curated Info

2

Fenton RA, et al. (2017) Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis. Kidney Int 92, 397-414
28385297   Curated Info

3

Chen Y, Wu S, Tian Y, Kong J (2017) Phosphorylation and subcellular localization of Na(+)/H(+) exchanger isoform 3 (NHE3) are associated with altered gallbladder absorptive function after formation of cholesterol gallstones. J Physiol Biochem 73, 133-139
27822917   Curated Info

4

Fenton RA, et al. (2015) Caffeine-induced diuresis and natriuresis is independent of renal tubular NHE3. Am J Physiol Renal Physiol 308, F1409-20
25925253   Curated Info

5

Chen T, et al. (2015) Cyclic GMP kinase II (cGKII) inhibits NHE3 by altering its trafficking and phosphorylating NHE3 at three required sites: identification of a multifunctional phosphorylation site. J Biol Chem 290, 1952-65
25480791   Curated Info

6

Rieg T, et al. (2012) Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice. Am J Physiol Renal Physiol 303, F963-71
22832924   Curated Info

7

Murtazina R, et al. (2011) NHERF2 is necessary for basal activity, second messenger inhibition, and LPA stimulation of NHE3 in mouse distal ileum. Am J Physiol Cell Physiol 301, C126-36
21430287   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info