Ser550

Site Information
AEGERRGsLAFIRSP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 450899

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 8 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 7 ) , western blotting ( 1 , 2 , 3 , 4 , 6 , 7 )
Relevant cell line - cell type - tissue:	'kidney, tubule' ( 2 ) , gallbladder ( 3 ) , ileum ( 7 ) , kidney ( 1 , 4 , 6 , 8 )

Upstream Regulation
Regulatory protein:	NHERF-2 (mouse) ( 7 )
Treatments:	alogliptin ( 6 ) , caffeine ( 4 ) , empagliflozin ( 1 ) , excendin-4 ( 6 ) , GLP-1 ( 6 ) , hypertonic_buffer ( 2 ) , lithogenic diet ( 3 ) , low Na ( 2 ) , NKH_477 ( 7 )

References
1	Onishi A, et al. (2020) A role for the tubular Na-H-exchanger NHE3 in the natriuretic effect of the SGLT2 inhibitor empagliflozin. Am J Physiol Renal Physiol 32893663 Curated Info
2	Fenton RA, et al. (2017) Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis. Kidney Int 92, 397-414 28385297 Curated Info
3	Chen Y, Wu S, Tian Y, Kong J (2017) Phosphorylation and subcellular localization of Na(+)/H(+) exchanger isoform 3 (NHE3) are associated with altered gallbladder absorptive function after formation of cholesterol gallstones. J Physiol Biochem 73, 133-139 27822917 Curated Info
4	Fenton RA, et al. (2015) Caffeine-induced diuresis and natriuresis is independent of renal tubular NHE3. Am J Physiol Renal Physiol 308, F1409-20 25925253 Curated Info
5	Chen T, et al. (2015) Cyclic GMP kinase II (cGKII) inhibits NHE3 by altering its trafficking and phosphorylating NHE3 at three required sites: identification of a multifunctional phosphorylation site. J Biol Chem 290, 1952-65 25480791 Curated Info
6	Rieg T, et al. (2012) Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice. Am J Physiol Renal Physiol 303, F963-71 22832924 Curated Info
7	Murtazina R, et al. (2011) NHERF2 is necessary for basal activity, second messenger inhibition, and LPA stimulation of NHE3 in mouse distal ileum. Am J Physiol Cell Physiol 301, C126-36 21430287 Curated Info
8	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info