Thr1479
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Home > Phosphorylation Site Page: > Thr1479  -  LRP6 (human)

Site Information
SSsstKGtyFPAILN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 457635

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , electrophoretic mobility shift ( 9 ) , immunoassay ( 5 ) , immunoprecipitation ( 5 ) , mass spectrometry ( 4 , 6 ) , mutation of modification site ( 9 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 7 , 8 , 9 ) , western blotting ( 1 , 2 , 3 , 5 , 7 )
Disease tissue studied:
breast cancer ( 5 ) , colorectal cancer ( 2 ) , gastric cancer ( 6 ) , gastric carcinoma ( 6 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 , 5 , 7 ) , fibroblast-embryo [CK1G1 (human)] ( 9 ) , fibroblast-embryo [LRP6 (human)] ( 9 ) , HEK293T (epithelial) [CK1G1 (human)] ( 9 ) , HEK293T (epithelial) [LRP6 (human)] ( 9 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) [DVL1 (human)] ( 8 ) , MCF-7 (breast cell) ( 5 ) , MKN-45 (gastric) ( 6 )

Upstream Regulation
Regulatory protein:
CK1G1 (human) ( 1 ) , CK1G2 (human) ( 1 ) , LRP6 (human) ( 3 ) , WTX (human) ( 5 )
Putative in vivo kinases:
CK1G1 (human) ( 9 ) , CK1G3 (human) ( 1 )
Kinases, in vitro:
CK1G1 (human) ( 9 )
Treatments:
FP1-24-2 ( 1 ) , PI(4,5)P2 ( 7 ) , siRNA ( 1 ) , TPO ( 2 ) , Wnt ( 8 , 9 ) , Wnt3a ( 1 , 5 , 7 )

Downstream Regulation
Effects of modification on LRP6:
activity, induced ( 9 ) , molecular association, regulation ( 8 )
Effects of modification on biological processes:
signaling pathway regulation ( 1 ) , transcription, induced ( 1 )
Induce interaction with:
axin 1 (human) ( 8 )

References 

1

Agajanian MJ, et al. (2022) Protein proximity networks and functional evaluation of the casein kinase 1 gamma family reveal unique roles for CK1γ3 in WNT signaling. J Biol Chem 298, 101986
35487243   Curated Info

2

Wu Z, et al. (2015) TPO-Induced Metabolic Reprogramming Drives Liver Metastasis of Colorectal Cancer CD110+ Tumor-Initiating Cells. Cell Stem Cell 17, 47-59
26140605   Curated Info

3

Vinyoles M, et al. (2014) Multivesicular GSK3 sequestration upon Wnt signaling is controlled by p120-catenin/cadherin interaction with LRP5/6. Mol Cell 53, 444-57
24412065   Curated Info

4

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

5

Tanneberger K, et al. (2011) Amer1/WTX couples Wnt-induced formation of PtdIns(4,5)P2 to LRP6 phosphorylation. EMBO J 30, 1433-43
21304492   Curated Info

6

Moritz A (2010) CST Curation Set: 10617; Year: 2010; Biosample/Treatment: cell line, MKN-45/DMSO; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

7

Pan W, et al. (2008) Wnt3a-mediated formation of phosphatidylinositol 4,5-bisphosphate regulates LRP6 phosphorylation. Science 321, 1350-3
18772438   Curated Info

8

Bilic J, et al. (2007) Wnt induces LRP6 signalosomes and promotes dishevelled-dependent LRP6 phosphorylation. Science 316, 1619-22
17569865   Curated Info

9

Davidson G, et al. (2005) Casein kinase 1 gamma couples Wnt receptor activation to cytoplasmic signal transduction. Nature 438, 867-72
16341016   Curated Info

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