Ser446

Site Information
DsIRLQIsNPDLKDR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 26389901

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 3 ) , mutation of modification site ( 2 , 3 , 4 ) , phospho-antibody ( 2 ) , western blotting ( 2 , 3 )
Disease tissue studied:	HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 ) , leukemia ( 2 ) , fibrosarcoma of soft tissue ( 3 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 3 ) , breast ( 1 ) , E.coli (bacterial) ( 4 ) , Gen2.2 (dendritic cell) ( 2 ) , HT1080 (fibroblast) ( 3 )

Downstream Regulation
Effects of modification on IRF5:	intracellular localization ( 2 , 3 ) , molecular association, regulation ( 4 ) , protein stabilization ( 4 )
Effects of modification on biological processes:	apoptosis, induced ( 3 ) , transcription, induced ( 3 )
Induce interaction with:	CBP (human) ( 4 )

References
1	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
2	Lopez-Pelaez M, et al. (2014) Protein kinase IKKβ-catalyzed phosphorylation of IRF5 at Ser462 induces its dimerization and nuclear translocation in myeloid cells. Proc Natl Acad Sci U S A 111, 17432-7 25326418 Curated Info
3	Chang Foreman HC, Van Scoy S, Cheng TF, Reich NC (2012) Activation of interferon regulatory factor 5 by site specific phosphorylation. PLoS One 7, e33098 22412986 Curated Info
4	Chen W, et al. (2008) Insights into interferon regulatory factor activation from the crystal structure of dimeric IRF5. Nat Struct Mol Biol 15, 1213-20 18836453 Curated Info