Ser215

Site Information
EsLSTHtsPsQsPNs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 457605

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , phospho-antibody ( 6 ) , western blotting ( 6 )
Disease tissue studied:	breast cancer ( 1 ) , neuroblastoma ( 6 )
Relevant cell line - cell type - tissue:	293 (epithelial) [MOR-1 (human)] ( 6 ) , 293E (epithelial) ( 2 ) , HeLa (cervical) ( 3 , 4 , 5 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 1 ) , HMLER ('stem, breast cancer') ( 1 ) , SH-SY5Y (neural crest) ( 6 )

Upstream Regulation
Putative in vivo kinases:	P38A (human) ( 6 )
Kinases, in vitro:	P38A (human) ( 6 )

References
1	Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90 24782546 Curated Info
2	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
3	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
4	Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8 19276368 Curated Info
5	Cantin GT, et al. (2008) Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res 7, 1346-51 18220336 Curated Info
6	Macé G, Miaczynska M, Zerial M, Nebreda AR (2005) Phosphorylation of EEA1 by p38 MAP kinase regulates mu opioid receptor endocytosis. EMBO J 24, 3235-46 16138080 Curated Info