Ser2
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Home > Phosphorylation Site Page: > Ser2  -  CK2B (human)

Site Information
______MsssEEVsW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447646

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 8 ) , mutation of modification site ( 7 ) , peptide sequencing ( 8 ) , phosphoamino acid analysis ( 9 ) , phosphopeptide mapping ( 9 )
Disease tissue studied:
cervical cancer ( 5 ) , cervical adenocarcinoma ( 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 3 ) , A431 (epithelial) ( 9 ) , A498 (renal) ( 4 ) , COS (fibroblast) ( 7 ) , Flp-In T-Rex-293 (epithelial) ( 1 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 1 ) , HeLa S3 (cervical) ( 5 , 6 ) , HUES-9 ('stem, embryonic') ( 2 ) , liver ( 8 ) , U2OS (bone cell) [GR (human)] ( 7 )

Upstream Regulation
Kinases, in vitro:
CK2A1 (human) ( 9 ) , CK2A2 (human) ( 9 )

Downstream Regulation
Effects of modification on CK2B:
enzymatic activity, induced ( 8 ) , protein stabilization ( 7 )
Induce interaction with:
CK2B (human) ( 8 )

References 

1

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

2

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

3

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

4

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

5

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

6

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

7

Zhang C, Vilk G, Canton DA, Litchfield DW (2002) Phosphorylation regulates the stability of the regulatory CK2beta subunit. Oncogene 21, 3754-64
12032843   Curated Info

8

Boldyreff B, James P, Staudenmann W, Issinger OG (1993) Ser2 is the autophosphorylation site in the beta subunit from bicistronically expressed human casein kinase-2 and from native rat liver casein kinase-2 beta. Eur J Biochem 218, 515-21
8269941   Curated Info

9

Litchfield DW, et al. (1991) Phosphorylation of the beta subunit of casein kinase II in human A431 cells. Identification of the autophosphorylation site and a site phosphorylated by p34cdc2. J Biol Chem 266, 20380-9
1939094   Curated Info