Tyr1284
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Home > Phosphorylation Site Page: > Tyr1284  -  HER4 (human)

Site Information
IVAENPEyLSEFSLK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 457470
Available spectra:  1 CST
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 5 ) , immunoprecipitation ( 5 ) , mass spectrometry ( 2 , 4 , 6 , 7 , 8 , 9 , 11 ) , mutation of modification site ( 5 ) , phospho-antibody ( 1 , 3 , 5 ) , western blotting ( 3 , 5 )
Disease tissue studied:
breast cancer ( 3 , 11 ) , breast adenocarcinoma ( 11 ) , gastric cancer ( 7 ) , lung cancer ( 2 , 3 , 9 ) , non-small cell lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 ) , non-small cell large cell lung carcinoma ( 2 ) , ovarian cancer ( 4 , 8 ) , melanoma skin cancer ( 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
HER4 (human) ( 5 )
Treatments:
lapatinib ( 5 ) , phorbol_ester ( 1 ) , Trametinib ( 1 )

Downstream Regulation
Effects of modification on HER4:
enzymatic activity, induced ( 5 ) , molecular association, regulation ( 10 )
Effects of modification on biological processes:
apoptosis, inhibited ( 5 ) , cell growth, induced ( 5 )
Induce interaction with:
SHC1 (human) ( 10 )

References 

1

Haryuni RD, et al. (2019) Negative feedback regulation of ErbB4 tyrosine kinase activity by ERK-mediated non-canonical phosphorylation. Biochem Biophys Res Commun 514, 456-461
31053301   Curated Info

2

Rikova K, Hall B (2013) CST Curation Set: 20740, 21167, 30170, 30171, 30172; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

3

Chandarlapaty S, et al. (2011) AKT inhibition relieves feedback suppression of receptor tyrosine kinase expression and activity. Cancer Cell 19, 58-71
21215704   Curated Info

4

Ren H (2010) CST Curation Set: 10743; Year: 2010; Biosample/Treatment: cell line, OVKATE/serum starved; Disease: ovarian cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Prickett TD, et al. (2009) Analysis of the tyrosine kinome in melanoma reveals recurrent mutations in ERBB4. Nat Genet 41, 1127-32
19718025   Curated Info

6

Kaushansky A, et al. (2008) System-wide investigation of ErbB4 reveals 19 sites of Tyr phosphorylation that are unusually selective in their recruitment properties. Chem Biol 15, 808-17
18721752   Curated Info

7

Ren H (2008) CST Curation Set: 3915; Year: 2008; Biosample/Treatment: cell line, AZ-521/untreated; Disease: gastric cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Guo A (2006) CST Curation Set: 1544; Year: 2006; Biosample/Treatment: cell line, EFO-21/serum starved; Disease: ovarian cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Mitchell J (2005) CST Curation Set: 827; Year: 2005; Biosample/Treatment: cell line, NCI-H661/serum starved; Disease: lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Schulze WX, Deng L, Mann M (2005) Phosphotyrosine interactome of the ErbB-receptor kinase family. Mol Syst Biol 1, 2005.0008
16729043   Curated Info

11

Moritz A (2003) CST Curation Set: 222; Year: 2003; Biosample/Treatment: cell line, MCF-7/NRG1; Disease: breast adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info