Ser554
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.8.0
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser554  -  NHE3 (rabbit)

Site Information
TEGERRGsLAFIRSP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450899

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 2 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
colorectal cancer ( 1 ) , colorectal carcinoma ( 1 )
Relevant cell line - cell type - tissue:
C2BBe1 (intestinal) ( 1 ) , ileum ( 1 ) , PS120 (fibroblast) ( 1 , 2 )

Upstream Regulation
Putative in vivo kinases:
CK2A1 (human) ( 2 ) , PKG2 (human) ( 1 )
Kinases, in vitro:
PKG2 (human) ( 1 )
Treatments:
8pCPT-cGMP ( 1 ) , cAMP_analog ( 1 ) , cGMP_analog ( 1 ) , H-89 ( 1 ) , NKH_477 ( 1 )

Downstream Regulation
Effects of modification on NHE3:
activity, inhibited ( 1 ) , intracellular localization ( 1 )

References 

1

Chen T, et al. (2015) Cyclic GMP kinase II (cGKII) inhibits NHE3 by altering its trafficking and phosphorylating NHE3 at three required sites: identification of a multifunctional phosphorylation site. J Biol Chem 290, 1952-65
25480791   Curated Info

2

Sarker R, et al. (2008) Casein kinase 2 binds to the C terminus of Na+/H+ exchanger 3 (NHE3) and stimulates NHE3 basal activity by phosphorylating a separate site in NHE3. Mol Biol Cell 19, 3859-70
18614797   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2024 Cell Signaling Technology, Inc.