Ser916
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Home > Phosphorylation Site Page: > Ser916  -  PRKD1 (mouse)

Site Information
KALSERVsIL_____   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447632

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 20 ) , mass spectrometry ( 4 , 7 , 9 , 19 ) , mutation of modification site ( 3 , 13 , 16 , 19 , 20 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 6 , 8 , 10 , 11 , 12 , 14 , 15 , 16 , 17 , 18 , 20 ) , western blotting ( 1 , 2 , 5 , 6 , 8 , 10 , 11 , 12 , 14 , 15 , 17 , 18 , 20 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 2 ) , 3T3 (fibroblast) ( 13 ) , A20 (B lymphocyte) ( 16 , 20 ) , aorta ( 2 ) , COS (fibroblast) ( 20 ) , heart ( 6 ) , HEK293T (epithelial) ( 8 , 19 ) , lymphocyte ( 1 ) , MEF (fibroblast) ( 7 , 8 , 15 ) , myocyte-heart ( 5 , 14 ) , neuron-'brain, hippocampus' ( 3 ) , spleen ( 9 ) , stromal ( 4 ) , Swiss 3T3 (fibroblast) ( 12 , 17 , 18 ) , T lymphocyte-lymph node [PRKD1 (mouse), genetic knockin] ( 10 ) , T lymphocyte-lymph node [PRKD2 (mouse), genetic knockin] ( 10 ) , thymocyte [CBL (mouse), homozygous knockout] ( 11 )

Upstream Regulation
Regulatory protein:
EDG4 (human) ( 2 ) , LPAR1 (human) ( 2 ) , PTPRA (mouse) ( 15 )
Putative in vivo kinases:
PRKD1 (mouse) ( 20 )
Kinases, in vitro:
PRKD1 (mouse) ( 10 , 19 , 20 ) , PRKD2 (mouse) ( 10 )
Treatments:
antibody ( 1 , 11 , 20 ) , autocamtide_inhibitory_peptide ( 6 ) , BMS-502 ( 1 ) , bombesin ( 12 , 17 , 18 ) , cytochalasin_D ( 18 ) , EGF ( 18 ) , endothelin ( 5 , 14 ) , GF109203X ( 12 , 18 ) , Go_6983 ( 12 ) , H2O2 ( 15 ) , HA-1077 ( 18 ) , insulin ( 18 ) , isoproterenol ( 5 ) , K252a ( 12 ) , KCl ( 3 ) , LPA ( 2 ) , NKH_477 ( 5 ) , OVA ( 10 ) , PD98059 ( 18 ) , PDGF ( 12 ) , phenylephrine ( 5 ) , phorbol_ester ( 5 , 12 , 18 , 20 ) , PP2 ( 18 ) , rapamycin ( 18 ) , Ro31-8220 ( 18 ) , SB202190 ( 18 ) , thrombin ( 8 ) , vasopressin ( 12 , 17 , 18 ) , wortmannin ( 18 )

Downstream Regulation
Effects of modification on PRKD1:
enzymatic activity, induced ( 5 , 20 ) , molecular association, regulation ( 13 ) , protein conformation ( 19 )
Effects of modification on biological processes:
cell motility, altered ( 13 ) , cytoskeletal reorganization ( 13 )
Induce interaction with:
ITGB1 (mouse) ( 13 )

References 

1

Wichroski M, et al. (2023) DGKα/ζ inhibitors combine with PD-1 checkpoint therapy to promote T cell-mediated antitumor immunity. Sci Transl Med 15, eadh1892
37878674   Curated Info

2

Hao F, et al. (2021) LPA-mediated PKD2 activation promotes LPA-induced tissue factor expression via the p38α and JNK2 MAPK pathways in smooth muscle cells. J Biol Chem, 101152
34478715   Curated Info

3

Bencsik N, et al. (2015) Protein kinase D promotes plasticity-induced F-actin stabilization in dendritic spines and regulates memory formation. J Cell Biol 210, 771-83
26304723   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Nichols CB, et al. (2014) β-Adrenergic Signaling Inhibits Gq-Dependent Protein Kinase D Activation by Preventing Protein Kinase D Translocation. Circ Res 114, 1398-409
24643961   Curated Info

6

Chakraborty A, et al. (2014) Inhibition of CaMKII Does Not Attenuate Cardiac Hypertrophy in Mice with Dysfunctional Ryanodine Receptor. PLoS One 9, e104338
25093823   Curated Info

7

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

8

Hatziapostolou M, et al. (2011) Tumor progression locus 2 mediates signal-induced increases in cytoplasmic calcium and cell migration. Sci Signal 4, ra55
21868363   Curated Info

9

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

10

Matthews SA, et al. (2010) Unique functions for protein kinase D1 and protein kinase D2 in mammalian cells. Biochem J 432, 153-63
20819079   Curated Info

11

Thien CB, et al. (2010) c-Cbl promotes T cell receptor-induced thymocyte apoptosis by activating the phosphatidylinositol 3-kinase/Akt pathway. J Biol Chem 285, 10969-81
20133944   Curated Info

12

Sinnett-Smith J, et al. (2009) Protein Kinase D Mediates Mitogenic Signaling by Gq-coupled Receptors through Protein Kinase C-independent Regulation of Activation Loop Ser744 and Ser748 Phosphorylation. J Biol Chem 284, 13434-45
19289471   Curated Info

13

White DP, Caswell PT, Norman JC (2007) alpha v beta3 and alpha5beta1 integrin recycling pathways dictate downstream Rho kinase signaling to regulate persistent cell migration. J Cell Biol 177, 515-25
17485491   Curated Info

14

Cuello F, et al. (2007) Protein kinase D selectively targets cardiac troponin I and regulates myofilament Ca2+ sensitivity in ventricular myocytes. Circ Res 100, 864-73
17322173   Curated Info

15

Hao Q, Rutherford SA, Low B, Tang H (2006) Selective regulation of hydrogen peroxide signaling by receptor tyrosine phosphatase-alpha. Free Radic Biol Med 41, 302-10
16814111   Curated Info

16

Wood CD, Marklund U, Cantrell DA (2005) Dual phospholipase C/diacylglycerol requirement for protein kinase D1 activation in lymphocytes. J Biol Chem 280, 6245-51
15590638   Curated Info

17

Sinnett-Smith J, et al. (2004) Protein kinase D potentiates DNA synthesis induced by Gq-coupled receptors by increasing the duration of ERK signaling in swiss 3T3 cells. J Biol Chem 279, 16883-93
14963034   Curated Info

18

Zhukova E, Sinnett-Smith J, Rozengurt E (2001) Protein kinase D potentiates DNA synthesis and cell proliferation induced by bombesin, vasopressin, or phorbol esters in Swiss 3T3 cells. J Biol Chem 276, 40298-305
11514571   Curated Info

19

Vertommen D, et al. (2000) Regulation of protein kinase D by multisite phosphorylation. Identification of phosphorylation sites by mass spectrometry and characterization by site-directed mutagenesis. J Biol Chem 275, 19567-76
10867018   Curated Info

20

Matthews SA, Rozengurt E, Cantrell D (1999) Characterization of serine 916 as an in vivo autophosphorylation site for protein kinase D/Protein kinase Cmu. J Biol Chem 274, 26543-9
10473617   Curated Info