Ser89
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser89  -  p300 (human)

Site Information
SELLRSGsSPNLNMG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447592

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 , 7 , 8 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 3 , 8 ) , mutation of modification site ( 1 , 3 , 5 , 6 , 7 , 8 ) , peptide sequencing ( 8 ) , phospho-antibody ( 2 , 5 , 6 ) , phosphopeptide mapping ( 8 ) , western blotting ( 2 , 3 , 6 )
Disease tissue studied:
colorectal cancer ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (human) ( 4 , 7 ) , PKCA (human) ( 8 ) , PKCD (human) ( 6 )
Kinases, in vitro:
AMPKA1 (human) ( 7 ) , PKCD (human) ( 6 )
Treatments:
acadesine ( 4 , 7 ) , calphostin_C ( 6 , 8 ) , Go_6976 ( 6 ) , metformin ( 4 ) , phenylephrine ( 5 ) , phorbol_ester ( 6 ) , Ro31-8220 ( 2 ) , rottlerin ( 6 ) , TPO ( 2 )

Downstream Regulation
Effects of modification on p300:
acetylation ( 2 ) , enzymatic activity, inhibited ( 4 , 6 ) , molecular association, regulation ( 2 , 7 )
Effects of modification on biological processes:
transcription, induced ( 5 ) , transcription, inhibited ( 4 , 6 , 8 )
Induce interaction with:
LRP6 (human) ( 2 )
Inhibit interaction with:
PPAR-gamma (mouse) ( 7 ) , RARG (human) ( 7 ) , RXRA (human) ( 7 ) , TR-beta1 (human) ( 7 )

References 

1

Sin TK, et al. (2020) Cancer-induced muscle wasting requires p38beta MAPK activation of p300. Cancer Res
33355181   Curated Info

2

Wu Z, et al. (2015) TPO-Induced Metabolic Reprogramming Drives Liver Metastasis of Colorectal Cancer CD110+ Tumor-Initiating Cells. Cell Stem Cell 17, 47-59
26140605   Curated Info

3

Ma H, Guo M, Shan B, Xia Z (2012) Targeted functional analysis of p300 coactivator in Wnt/β-catenin signaling pathway using phosphoproteomic and biochemical approaches. J Proteomics 75, 2601-10
22465714   Curated Info

4

Zhang Y, et al. (2011) AMP-activated protein kinase suppresses endothelial cell inflammation through phosphorylation of transcriptional coactivator p300. Arterioscler Thromb Vasc Biol 31, 2897-908
21940946   Curated Info

5

Gusterson RJ, Yuan LW, Latchman DS (2004) Distinct serine residues in CBP and p300 are necessary for their activation by phenylephrine. Int J Biochem Cell Biol 36, 893-9
15006641   Curated Info

6

Yuan LW, Soh JW, Weinstein IB (2002) Inhibition of histone acetyltransferase function of p300 by PKCdelta. Biochim Biophys Acta 1592, 205-11
12379484   Curated Info

7

Yang W, et al. (2001) Regulation of transcription by AMP-activated protein kinase: phosphorylation of p300 blocks its interaction with nuclear receptors. J Biol Chem 276, 38341-4
11518699   Curated Info

8

Yuan LW, Gambee JE (2000) Phosphorylation of p300 at serine 89 by protein kinase C. J Biol Chem 275, 40946-51
11020388   Curated Info