Ser1418

Site Information
sPEVKARsQsGtLDG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 457286

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , mutation of modification site ( 4 ) , phospho-antibody ( 5 ) , western blotting ( 5 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 4 ) , 293E (epithelial) ( 5 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 2 ) , Flp-In T-Rex-293 (epithelial) ( 2 ) , Jurkat (T lymphocyte) ( 3 ) , K562 (erythroid) ( 1 )

Upstream Regulation
Treatments:	phorbol_ester ( 5 )

References
1	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
2	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
3	Zhou J (2011) CST Curation Set: 12496; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
4	Nellist M, et al. (2005) Phosphorylation and binding partner analysis of the TSC1-TSC2 complex. Biochem Biophys Res Commun 333, 818-26 15963462 Curated Info
5	Ballif BA, et al. (2005) Quantitative phosphorylation profiling of the ERK/p90 ribosomal S6 kinase-signaling cassette and its targets, the tuberous sclerosis tumor suppressors. Proc Natl Acad Sci U S A 102, 667-72 15647351 Curated Info