Ser221
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Home > Phosphorylation Site Page: > Ser221  -  GTF2F1 (human)

Site Information
LEMssDAsDAsGEEG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456995

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 )
Disease tissue studied:
breast cancer ( 2 , 5 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , cervical cancer ( 14 ) , cervical adenocarcinoma ( 14 ) , leukemia ( 9 ) , acute myelogenous leukemia ( 9 ) , lung cancer ( 6 ) , non-small cell lung cancer ( 6 ) , lymphoma ( 3 ) , Burkitt's lymphoma ( 3 ) , follicular lymphoma ( 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 12 ) , 293E (epithelial) ( 10 ) , A498 (renal) ( 13 ) , BJAB (B lymphocyte) ( 3 ) , breast ( 1 ) , Calu 6 (pulmonary) ( 6 ) , Flp-In T-Rex-293 (epithelial) ( 7 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 7 ) , H2009 (pulmonary) ( 6 ) , H2077 (pulmonary) ( 6 ) , H2887 (pulmonary) ( 6 ) , H322 (pulmonary) ( 6 ) , H322M (pulmonary) ( 6 ) , HCC2279 (pulmonary) ( 6 ) , HCC366 (pulmonary) ( 6 ) , HCC78 (pulmonary) ( 6 ) , HCC827 (pulmonary) ( 6 ) , HeLa (cervical) ( 15 , 18 , 19 , 20 ) , HeLa S3 (cervical) ( 14 ) , HepG2 (hepatic) ( 21 ) , HMLER ('stem, breast cancer') ( 2 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 2 ) , HOP62 (pulmonary) ( 6 ) , HT-29 (intestinal) ( 22 ) , HUES-9 ('stem, embryonic') ( 11 ) , Jurkat (T lymphocyte) ( 4 , 16 , 17 ) , K562 (erythroid) ( 15 ) , KG-1 (myeloid) ( 9 ) , LCLC-103H (pulmonary) ( 6 ) , LOU-NH91 (squamous) ( 6 ) , NCI-H1395 (pulmonary) ( 6 ) , NCI-H1568 (pulmonary) ( 6 ) , NCI-H1648 (pulmonary) ( 6 ) , NCI-H2030 (pulmonary) ( 6 ) , NCI-H2172 (pulmonary) ( 6 ) , NCI-H460 (pulmonary) ( 6 ) , NCI-H647 (pulmonary) ( 6 ) , SKBr3 (breast cell) ( 5 ) , SU-DHL-4 (B lymphocyte) ( 3 )

Upstream Regulation
Treatments:
EGF ( 20 ) , nocodazole ( 14 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

3

Rolland D, et al. (2014) Global phosphoproteomic profiling reveals distinct signatures in B-cell non-Hodgkin lymphomas. Am J Pathol 184, 1331-42
24667141   Curated Info

4

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

5

Imami K, et al. (2012) Temporal profiling of lapatinib-suppressed phosphorylation signals in EGFR/HER2 pathways. Mol Cell Proteomics 11, 1741-57
22964224   Curated Info

6

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

7

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

8

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

9

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

10

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

11

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

12

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

13

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

14

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

15

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
19651622   Curated Info

16

Guo A (2009) CST Curation Set: 8004; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

17

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

18

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

19

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

20

Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 127, 635-48
17081983   Curated Info

21

Gevaert K, et al. (2005) Global phosphoproteome analysis on human HepG2 hepatocytes using reversed-phase diagonal LC. Proteomics 5, 3589-99
16097034   Curated Info

22

Kim JE, Tannenbaum SR, White FM (2005) Global phosphoproteome of HT-29 human colon adenocarcinoma cells. J Proteome Res 4, 1339-46
16083285   Curated Info