Ser181
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Home > Phosphorylation Site Page: > Ser181  -  SOX9 (human)

Site Information
YQPRRRKsVKNGQAE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456981

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , immunoprecipitation ( 2 , 10 ) , mutation of modification site ( 1 , 2 , 7 , 8 , 10 ) , phospho-antibody ( 1 , 5 , 6 , 7 , 8 , 9 , 10 ) , western blotting ( 1 , 2 , 5 , 6 , 7 , 8 )
Disease tissue studied:
bone cancer ( 6 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 ) , testicular cancer ( 8 )
Relevant cell line - cell type - tissue:
'stem, mesenchymal' ( 5 ) , 3T3 (fibroblast) ( 8 ) , A549 (pulmonary) ( 1 ) , ATDC5 (chondrocyte) ( 2 ) , chondrocyte ( 2 , 6 ) , chondrocyte-embryo ( 7 ) , COS (fibroblast) ( 9 , 10 ) , gonad ( 8 ) , NCI-H1299 (pulmonary) ( 1 ) , NT2 (testicular) ( 8 ) , SW1353 ( 6 )

Upstream Regulation
Regulatory protein:
PTHR (human) ( 9 ) , RHOA (human) ( 6 )
Putative in vivo kinases:
ADCK5 (human) ( 1 ) , PKACA (human) ( 7 , 8 , 10 ) , ROCK1 (human) ( 6 )
Kinases, in vitro:
PKACA (human) ( 8 , 10 ) , ROCK1 (human) ( 6 )
Treatments:
BW245C ( 8 ) , cAMP_analog ( 8 , 10 ) , FGF2 ( 5 ) , H-89 ( 7 , 8 , 9 ) , hydroxyfasudil ( 6 ) , okadaic_acid ( 9 ) , PGD2 ( 8 ) , PTHrP ( 9 ) , siRNA ( 1 ) , tamoxifen ( 6 ) , TGF-beta ( 6 )

Downstream Regulation
Effects of modification on SOX9:
activity, induced ( 6 , 10 ) , intracellular localization ( 1 , 6 , 8 ) , molecular association, regulation ( 8 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 ) , cell differentiation, induced ( 5 ) , cell motility, induced ( 1 ) , cytoskeletal reorganization ( 7 ) , signaling pathway regulation ( 1 ) , transcription, induced ( 1 , 6 , 7 )
Induce interaction with:
KPNB1 (human) ( 8 )

References 

1

Qiu M, et al. (2020) aarF domain containing kinase 5 gene promotes invasion and migration of lung cancer cells through ADCK5-SOX9-PTTG1 pathway. Exp Cell Res 392, 112002
32277958   Curated Info

2

Coricor G, Serra R (2016) TGF-β regulates phosphorylation and stabilization of Sox9 protein in chondrocytes through p38 and Smad dependent mechanisms. Sci Rep 6, 38616
27929080   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

5

Handorf AM, Li WJ (2011) Fibroblast growth factor-2 primes human mesenchymal stem cells for enhanced chondrogenesis. PLoS One 6, e22887
21818404   Curated Info

6

Haudenschild DR, et al. (2010) Rho kinase-dependent activation of SOX9 in chondrocytes. Arthritis Rheum 62, 191-200
20039424   Curated Info

7

Kumar D, Lassar AB (2009) The transcriptional activity of Sox9 in chondrocytes is regulated by RhoA signaling and actin polymerization. Mol Cell Biol 29, 4262-73
19470758   Curated Info

8

Malki S, et al. (2005) Prostaglandin D2 induces nuclear import of the sex-determining factor SOX9 via its cAMP-PKA phosphorylation. EMBO J 24, 1798-809
15889150   Curated Info

9

Huang W, Chung UI, Kronenberg HM, de Crombrugghe B (2001) The chondrogenic transcription factor Sox9 is a target of signaling by the parathyroid hormone-related peptide in the growth plate of endochondral bones. Proc Natl Acad Sci U S A 98, 160-5
11120880   Curated Info

10

Huang W, Zhou X, Lefebvre V, de Crombrugghe B (2000) Phosphorylation of SOX9 by cyclic AMP-dependent protein kinase A enhances SOX9's ability to transactivate a Col2a1 chondrocyte-specific enhancer. Mol Cell Biol 20, 4149-58
10805756   Curated Info