Thr155
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Home > Phosphorylation Site Page: > Thr155  -  PAR-4 (rat)

Site Information
LREKRRStGVVNIPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456964

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 , 4 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 3 , 4 ) , phospho-antibody ( 3 , 4 ) , western blotting ( 4 )
Disease tissue studied:
adrenal cancer ( 1 , 2 ) , pheochromocytoma ( 1 , 2 ) , breast cancer ( 4 ) , leukemia ( 4 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 4 ) , lung cancer ( 4 ) , non-small cell lung cancer ( 4 ) , prostate cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 4 )
Kinases, in vitro:
PKACA (human) ( 4 )
Treatments:
cAMP_analog ( 4 ) , mPKI ( 4 ) , vincristine ( 4 )

Downstream Regulation
Effects of modification on biological processes:
apoptosis, induced ( 4 ) , transcription, altered ( 4 )

References 

1

Zhou J (2009) CST Curation Set: 6823; Year: 2009; Biosample/Treatment: cell line, PC-12/forskolin; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

2

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

3

Goswami A, et al. (2005) Binding and phosphorylation of par-4 by akt is essential for cancer cell survival. Mol Cell 20, 33-44
16209943   Curated Info

4

Gurumurthy S, Goswami A, Vasudevan KM, Rangnekar VM (2005) Phosphorylation of Par-4 by protein kinase A is critical for apoptosis. Mol Cell Biol 25, 1146-61
15657440   Curated Info