Tyr89
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Tyr89  -  p27Kip1 (human)

Site Information
GsLPEFyyRPPRPPK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456819

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 2 ) , mutation of modification site ( 1 , 2 , 4 , 5 , 6 ) , phospho-antibody ( 3 , 4 , 5 ) , western blotting ( 3 , 4 , 5 )
Disease tissue studied:
lung cancer ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
Src (human) ( 5 )
Kinases, in vitro:
Abl (human) ( 2 , 4 ) , Src (human) ( 5 ) , Yes (human) ( 5 )

Downstream Regulation
Effects of modification on p27Kip1:
activity, induced ( 2 ) , activity, inhibited ( 4 ) , intracellular localization ( 6 ) , molecular association, regulation ( 6 )
Effects of modification on biological processes:
cell cycle regulation ( 2 , 4 )
Induce interaction with:
CDK2 (human) ( 4 ) , CDK4 (human) ( 4 , 6 )

References 

1

Ou L, Waddell MB, Kriwacki RW (2012) Mechanism of cell cycle entry mediated by the intrinsically disordered protein p27(Kip1). ACS Chem Biol 7, 678-82
22276948   Curated Info

2

Ou L, et al. (2011) Incomplete folding upon binding mediates Cdk4/cyclin D complex activation by tyrosine phosphorylation of inhibitor p27 protein. J Biol Chem 286, 30142-51
21715330   Curated Info

3

Lei K, Townsend DM, Tew KD (2008) Protein cysteine sulfinic acid reductase (sulfiredoxin) as a regulator of cell proliferation and drug response. Oncogene 27, 4877-87
18454177   Curated Info

4

James MK, Ray A, Leznova D, Blain SW (2008) Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol 28, 498-510
17908796   Curated Info

5

Chu I, et al. (2007) p27 phosphorylation by Src regulates inhibition of cyclin E-Cdk2. Cell 128, 281-94
17254967   Curated Info

6

Kardinal C, et al. (2006) Tyrosine phosphorylation modulates binding preference to cyclin-dependent kinases and subcellular localization of p27Kip1 in the acute promyelocytic leukemia cell line NB4. Blood 107, 1133-40
16195327   Curated Info