Tyr88
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Home > Phosphorylation Site Page: > Tyr88  -  p27Kip1 (human)

Site Information
kGsLPEFyyRPPRPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456818

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 , 4 ) , mutation of modification site ( 1 , 2 , 3 , 6 , 7 , 8 , 9 ) , phospho-antibody ( 2 , 4 , 5 , 6 , 7 , 8 ) , phosphoamino acid analysis ( 8 ) , western blotting ( 2 , 4 , 5 , 6 , 7 )
Disease tissue studied:
breast cancer ( 4 ) , leukemia ( 2 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 2 ) , lung cancer ( 5 ) , multiple myeloma ( 8 ) , fibrosarcoma of soft tissue ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
Abl (mouse) ( 1 , 8 ) , LYN (human) ( 8 ) , Src (human) ( 7 )
Kinases, in vitro:
Abl (human) ( 6 ) , Abl (mouse) ( 8 ) , LYN (mouse) ( 8 ) , Src (human) ( 7 ) , Yes (human) ( 7 )
Treatments:
GGTI-2417 ( 4 ) , imatinib ( 8 )

Downstream Regulation
Effects of modification on p27Kip1:
activity, inhibited ( 6 ) , intracellular localization ( 9 ) , molecular association, regulation ( 1 , 8 , 9 ) , phosphorylation ( 1 ) , protein conformation ( 1 ) , protein degradation ( 2 , 3 , 8 )
Effects of modification on biological processes:
cell cycle regulation ( 1 , 6 , 8 )
Induce interaction with:
CDK2 (human) ( 6 ) , CDK4 (human) ( 6 , 9 )
Inhibit interaction with:
CDK2 (human) ( 8 ) , CDK4 (human) ( 1 )

References 

1

Ou L, Waddell MB, Kriwacki RW (2012) Mechanism of cell cycle entry mediated by the intrinsically disordered protein p27(Kip1). ACS Chem Biol 7, 678-82
22276948   Curated Info

2

Jäkel H, Weinl C, Hengst L (2011) Phosphorylation of p27Kip1 by JAK2 directly links cytokine receptor signaling to cell cycle control. Oncogene 30, 3502-12
21423214   Curated Info

3

Schiappacassi M, et al. (2011) Role of T198 modification in the regulation of p27(Kip1) protein stability and function. PLoS One 6, e17673
21423803   Curated Info

4

Kazi A, et al. (2009) Blockade of protein geranylgeranylation inhibits Cdk2-dependent p27Kip1 phosphorylation on Thr187 and accumulates p27Kip1 in the nucleus: implications for breast cancer therapy. Mol Cell Biol 29, 2254-63
19204084   Curated Info

5

Lei K, Townsend DM, Tew KD (2008) Protein cysteine sulfinic acid reductase (sulfiredoxin) as a regulator of cell proliferation and drug response. Oncogene 27, 4877-87
18454177   Curated Info

6

James MK, Ray A, Leznova D, Blain SW (2008) Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity. Mol Cell Biol 28, 498-510
17908796   Curated Info

7

Chu I, et al. (2007) p27 phosphorylation by Src regulates inhibition of cyclin E-Cdk2. Cell 128, 281-94
17254967   Curated Info

8

Grimmler M, et al. (2007) Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cell 128, 269-80
17254966   Curated Info

9

Kardinal C, et al. (2006) Tyrosine phosphorylation modulates binding preference to cyclin-dependent kinases and subcellular localization of p27Kip1 in the acute promyelocytic leukemia cell line NB4. Blood 107, 1133-40
16195327   Curated Info