Ser491
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Home > Phosphorylation Site Page: > Ser491  -  AMPKA2 (mouse)

Site Information
StPQRSCsAAGLHRA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450432

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 , 4 , 5 , 6 ) , phospho-antibody ( 2 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 4 ) , C2C12 (myoblast) ( 2 ) , liver ( 1 , 3 , 5 , 6 ) , liver [leptin (mouse), homozygous knockout] ( 5 )

Upstream Regulation
Putative in vivo kinases:
PRKD1 (human) ( 2 )
Treatments:
CRT0066101 ( 2 ) , Go_6983 ( 2 ) , insulin ( 4 ) , LY294002 ( 4 ) , MK-2206 ( 4 ) , phorbol_ester ( 2 ) , rapamycin ( 2 ) , wortmannin ( 2 )

Downstream Regulation
Effects of modification on AMPKA2:
enzymatic activity, inhibited ( 2 )

References 

1

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

2

Coughlan KA, et al. (2016) PKD1 Inhibits AMPK╬▒2 through Phosphorylation of Serine 491 and Impairs Insulin Signaling in Skeletal Muscle Cells. J Biol Chem 291, 5664-75
26797128   Curated Info

3

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

4

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

5

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

6

Guo A (2007) CST Curation Set: 3227; Year: 2007; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info