Thr172
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Home > Phosphorylation Site Page: > Thr172  -  AMPKA2 (mouse)

Site Information
sDGEFLRtsCGsPNY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448026

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 , 8 ) , mass spectrometry ( 6 , 9 , 14 , 16 , 18 , 19 , 21 , 22 , 23 , 24 , 25 ) , mutation of modification site ( 3 , 4 , 8 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 7 , 8 , 12 , 13 , 15 , 17 , 20 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ) , western blotting ( 1 , 3 , 4 , 5 , 7 , 8 , 12 , 13 , 15 , 17 , 20 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 34 , 36 )
Disease tissue studied:
lung cancer ( 31 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 6 ) , 'muscle, skeletal' ( 13 , 28 , 33 , 34 ) , 'muscle, skeletal' [CAMKK1 (mouse)] ( 30 ) , 293 (epithelial) ( 4 ) , 3T3 (fibroblast) ( 8 ) , 3T3-L1 (fibroblast) ( 29 ) , AML12 (hepatic) ( 4 ) , brain ( 16 , 19 , 22 , 25 ) , C2C12 (myoblast) ( 13 , 29 ) , embryo ( 16 ) , GT1-7 (neuron) ( 17 ) , heart ( 24 , 27 , 32 , 34 ) , HEK293T (epithelial) ( 8 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 9 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 9 ) , HL-1 (myocyte) ( 9 ) , HUVEC (endothelial) ( 3 ) , L6 (myoblast) ( 29 ) , leukocyte-blood ( 1 ) , liver ( 14 , 16 , 18 , 21 , 23 , 26 , 36 ) , lung ( 31 ) , MEF (fibroblast) ( 8 , 15 , 32 ) , MEF (fibroblast) [IGF1R (mouse)] ( 35 ) , muscle ( 20 ) , myocyte-heart ( 12 , 32 ) , osteoblast-calvarium ( 5 ) , vascular smooth muscle cell ('muscle, smooth') ( 1 )

Upstream Regulation
Regulatory protein:
CAMKK1 (mouse) ( 30 ) , GLUT1 (mouse) ( 5 ) , HGK (mouse) ( 32 ) , LKB1 (mouse) ( 20 , 34 ) , SCD (mouse) ( 36 ) , SIRT1 (human) ( 26 ) , TAK1 (mouse) ( 32 )
Putative in vivo kinases:
AMPKA2 (human) ( 3 )
Treatments:
A-769662 ( 8 , 12 ) , acadesine ( 13 , 29 , 32 ) , adiponectin ( 29 ) , angiotensin_2 ( 1 , 3 ) , anoxia ( 34 ) , benzo(a)pyrene ( 31 ) , caffeine ( 28 ) , clozapine ( 2 ) , colforsin ( 4 ) , compound_C ( 1 , 12 ) , dantrolene ( 28 ) , exercise ( 7 , 11 ) , food deprivation ( 8 ) , glucose ( 5 , 17 ) , glucose_starvation ( 29 , 35 ) , H2O2 ( 3 ) , HG9-91-01 ( 4 ) , high_glucose ( 3 ) , insulin ( 30 ) , ischemia ( 32 , 34 ) , ischemia/reperfusion ( 27 ) , KN-93 ( 28 ) , leptin ( 29 ) , leucine_deprivation ( 8 ) , low_glucose ( 17 ) , metformin ( 3 , 8 , 27 , 32 ) , muscle contraction ( 30 ) , NTCU ( 31 ) , oligomycin ( 32 ) , pomegranate_wine ( 31 ) , pravastatin ( 1 ) , pterosin_B ( 4 ) , rapamycin ( 8 ) , STO-609 ( 28 , 30 ) , SU6656 ( 20 ) , telmisartan ( 3 ) , tempol ( 1 )

Downstream Regulation
Effects of modification on AMPKA2:
enzymatic activity, induced ( 1 , 5 , 29 , 35 ) , intracellular localization ( 29 )
Effects of modification on biological processes:
cell differentiation, inhibited ( 5 ) , transcription, altered ( 29 ) , transcription, induced ( 1 )

References 

1

Ma H, et al. (2017) Pravastatin activates activator protein 2 alpha to augment the angiotensin II-induced abdominal aortic aneurysms. Oncotarget 8, 14294-14305
28179583   Curated Info

2

Choi Y, et al. (2017) Clozapine Improves Memory Impairment and Reduces Aβ Level in the Tg-APPswe/PS1dE9 Mouse Model of Alzheimer's Disease. Mol Neurobiol 54, 450-460
26742522   Curated Info

3

Shang F, et al. (2016) Cardiovascular Protective Effect of Metformin and Telmisartan: Reduction of PARP1 Activity via the AMPK-PARP1 Cascade. PLoS One 11, e0151845
26986624   Curated Info

4

Itoh Y, et al. (2015) Salt-inducible Kinase 3 Signaling Is Important for the Gluconeogenic Programs in Mouse Hepatocytes. J Biol Chem 290, 17879-93
26048985   Curated Info

5

Wei J, et al. (2015) Glucose Uptake and Runx2 Synergize to Orchestrate Osteoblast Differentiation and Bone Formation. Cell 161, 1576-91
26091038   Curated Info

6

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

7

Knudsen JG, et al. (2015) Exercise-induced regulation of key factors in substrate choice and gluconeogenesis in mouse liver. Mol Cell Biochem 403, 209-17
25702176   Curated Info

8

Dai S, et al. (2015) Suppression of the HSF1-mediated proteotoxic stress response by the metabolic stress sensor AMPK. EMBO J 34, 275-93
25425574   Curated Info

9

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

10

Davies M, et al. (2014) Novel mechanisms of Na+ retention in obesity: phosphorylation of NKCC2 and regulation of SPAK/OSR1 by AMPK. Am J Physiol Renal Physiol 307, F96-F106
24808538   Curated Info

11

Pagano AF, et al. (2014) Autophagy and protein turnover signaling in slow-twitch muscle during exercise. Med Sci Sports Exerc 46, 1314-25
24389528   Curated Info

12

Chen S, et al. (2014) Alpha1 catalytic subunit of AMPK modulates contractile function of cardiomyocytes through phosphorylation of troponin I. Life Sci 98, 75-82
24447627   Curated Info

13

Yamada E, Bastie CC (2014) Disruption of Fyn SH3 Domain Interaction with a Proline-Rich Motif in Liver Kinase B1 Results in Activation of AMP-Activated Protein Kinase. PLoS One 9, e89604
24586906   Curated Info

14

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

15

Schröfelbauer B, et al. (2012) NEMO Ensures Signaling Specificity of the Pleiotropic IKKβ by Directing Its Kinase Activity toward IκBα. Mol Cell 47, 111-21
22633953   Curated Info

16

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

17

Bang S, et al. (2012) AMP-activated protein kinase is physiologically regulated by inositol polyphosphate multikinase. Proc Natl Acad Sci U S A 109, 616-20
22203993   Curated Info

18

Guo A (2011) CST Curation Set: 12478; Year: 2011; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

19

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

20

Yamada E, et al. (2010) Fyn-dependent regulation of energy expenditure and body weight is mediated by tyrosine phosphorylation of LKB1. Cell Metab 11, 113-24
20142099   Curated Info

21

Zhou J (2009) CST Curation Set: 7426; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
Curated Info

22

Zhou J (2009) CST Curation Set: 7413; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
Curated Info

23

Zhou J (2009) CST Curation Set: 7425; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
Curated Info

24

Zhou J (2009) CST Curation Set: 7418; Year: 2009; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
Curated Info

25

Zhou J (2009) CST Curation Set: 7414; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
Curated Info

26

Hou X, et al. (2008) SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase. J Biol Chem 283, 20015-26
18482975   Curated Info

27

Calvert JW, et al. (2008) Acute metformin therapy confers cardioprotection against myocardial infarction via AMPK-eNOS-mediated signaling. Diabetes 57, 696-705
18083782   Curated Info

28

Jensen TE, et al. (2007) Caffeine-induced Ca(2+) release increases AMPK-dependent glucose uptake in rodent soleus muscle. Am J Physiol Endocrinol Metab 293, E286-92
17405829   Curated Info

29

Suzuki A, et al. (2007) Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha2 form of AMP-activated protein kinase. Mol Cell Biol 27, 4317-27
17420279   Curated Info

30

Witczak CA, Fujii N, Hirshman MF, Goodyear LJ (2007) Ca2+/calmodulin-dependent protein kinase kinase-alpha regulates skeletal muscle glucose uptake independent of AMP-activated protein kinase and Akt activation. Diabetes 56, 1403-9
17287469   Curated Info

31

Khan N, et al. (2007) Oral consumption of pomegranate fruit extract inhibits growth and progression of primary lung tumors in mice. Cancer Res 67, 3475-82
17389758   Curated Info

32

Xie M, et al. (2006) A pivotal role for endogenous TGF-beta-activated kinase-1 in the LKB1/AMP-activated protein kinase energy-sensor pathway. Proc Natl Acad Sci U S A 103, 17378-83
17085580   Curated Info

33

Kramer HF, et al. (2006) AS160 regulates insulin- and contraction-stimulated glucose uptake in mouse skeletal muscle. J Biol Chem 281, 31478-85
16935857   Curated Info

34

Sakamoto K, et al. (2006) Deficiency of LKB1 in heart prevents ischemia-mediated activation of AMPKalpha2 but not AMPKalpha1. Am J Physiol Endocrinol Metab 290, E780-8
16332922   Curated Info

35

Jones RG, et al. (2005) AMP-activated protein kinase induces a p53-dependent metabolic checkpoint. Mol Cell 18, 283-93
15866171   Curated Info

36

Dobrzyn P, et al. (2004) Stearoyl-CoA desaturase 1 deficiency increases fatty acid oxidation by activating AMP-activated protein kinase in liver. Proc Natl Acad Sci U S A 101, 6409-14
15096593   Curated Info