Thr172
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Home > Phosphorylation Site Page: > Thr172  -  AMPKA2 (mouse)

Site Information
sDGEFLRtsCGsPNY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448026

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 7 , 16 , 18 , 23 ) , immunoprecipitation ( 31 , 36 ) , mass spectrometry ( 34 , 37 , 42 , 44 , 46 , 47 , 48 , 50 , 51 , 52 , 53 , 54 ) , mutation of modification site ( 10 , 31 , 32 , 36 ) , peptide sequencing ( 18 ) , phospho-antibody ( 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 29 , 31 , 32 , 33 , 35 , 36 , 38 , 39 , 40 , 41 , 43 , 45 , 49 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ) , western blotting ( 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 29 , 31 , 32 , 33 , 35 , 36 , 39 , 40 , 41 , 43 , 45 , 49 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 63 , 65 , 66 )
Disease tissue studied:
Alzheimer's disease ( 11 ) , breast cancer ( 12 ) , breast adenocarcinoma ( 12 ) , breast cancer, triple negative ( 12 ) , lung cancer ( 15 , 60 ) , non-small cell lung cancer ( 15 ) , non-small cell lung adenocarcinoma ( 15 ) , non-small cell large cell lung carcinoma ( 15 ) , neuroblastoma ( 13 ) , fibrosarcoma of soft tissue ( 18 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 34 ) , 'brain, cerebellum' ( 25 ) , 'brain, cerebral cortex' ( 11 ) , 'brain, hippocampus' ( 23 ) , 'fat, brown' ( 47 ) , 'kidney, cortex' ( 38 ) , 'muscle, skeletal' ( 24 , 39 , 41 , 57 , 62 , 63 ) , 'muscle, skeletal' [CAMKK1 (mouse)] ( 59 ) , 293 (epithelial) ( 18 , 32 ) , 3T3 (fibroblast) ( 8 , 36 ) , 3T3-L1 (fibroblast) ( 58 ) , 4T1 (breast cell) ( 12 ) , A549 (pulmonary) ( 15 ) , AML12 (hepatic) ( 32 ) , blood ( 25 ) , bone ( 7 ) , brain ( 44 , 47 , 48 , 51 , 54 ) , BT-549 (breast cell) ( 12 ) , C2C12 (myoblast) ( 14 , 20 , 21 , 22 , 41 , 58 ) , Calu 6 (pulmonary) ( 15 ) , Calu-1 (squamous) ( 15 ) , chondrocyte ( 7 ) , COR-L23 (pulmonary) ( 15 ) , embryo ( 44 ) , fibroblast-heart ( 6 ) , GT1-7 (neuron) ( 45 ) , heart ( 10 , 53 , 56 , 61 , 63 ) , HEK293T (epithelial) ( 36 ) , hepatocyte-liver ( 9 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 37 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 37 ) , HL-1 (myocyte) ( 37 ) , HUVEC (endothelial) ( 31 ) , kidney ( 10 , 47 ) , L6 (myoblast) ( 58 ) , L929 (fibroblast) ( 18 ) , leukocyte-blood ( 29 ) , liver ( 4 , 9 , 17 , 25 , 42 , 44 , 46 , 50 , 52 , 55 , 65 , 66 ) , lung ( 15 , 16 , 25 , 26 , 47 , 60 ) , macrophage-peritoneum ( 26 ) , MCF-7 (breast cell) ( 12 ) , MDA-MB-231 (breast cell) ( 12 ) , MDA-MB-468 (breast cell) ( 12 ) , med1-MB ( 25 ) , MEF (fibroblast) [IGF1R (mouse)] ( 64 ) , MEF (fibroblast) ( 8 , 18 , 36 , 43 , 61 ) , muscle ( 10 , 19 , 49 ) , myocyte-heart ( 6 , 40 , 61 ) , myotubes ( 14 ) , NCI-H1299 (pulmonary) ( 15 ) , Neuro-2a (neuron) ( 13 ) , neuron ( 23 ) , osteoblast-calvarium ( 33 ) , pancreas ( 47 ) , spleen ( 24 , 47 ) , testis ( 47 ) , vascular smooth muscle cell ('muscle, smooth') ( 29 )

Upstream Regulation
Regulatory protein:
ASB2 (mouse) ( 19 ) , ATG14 (human) ( 24 ) , beclin 1 (human) ( 24 ) , CAMKK1 (mouse) ( 59 ) , Cas-L (human) ( 16 ) , DOK3 (mouse) ( 26 ) , FAK (human) ( 8 ) , GLUT1 (mouse) ( 33 ) , HGK (mouse) ( 61 ) , ITGAV (human) ( 8 ) , KRas (human) ( 15 ) , LDH-B (human) ( 15 ) , LKB1 (mouse) ( 49 , 63 ) , RIPK3 (human) ( 18 ) , SCD (mouse) ( 65 ) , SGK1 (human) ( 9 ) , SGK1 (mouse) ( 9 ) , SIRT1 (human) ( 55 ) , STING (human) ( 6 ) , TAK1 (mouse) ( 61 ) , TLR9 (human) ( 24 ) , ULK1 (human) ( 13 ) , UVRAG (human) ( 24 )
Putative in vivo kinases:
AMPKA2 (human) ( 31 ) , LKB1 (human) ( 7 ) , RIPK3 (human) ( 18 )
Treatments:
2-deoxyglucose ( 23 ) , A-769662 ( 36 , 40 ) , acadesine ( 41 , 58 , 61 ) , adiponectin ( 58 ) , angiotensin_2 ( 29 , 31 ) , anoxia ( 63 ) , benzo(a)pyrene ( 60 ) , berberine ( 7 ) , betulinic_acid ( 21 ) , birinapant ( 18 ) , caffeine ( 57 ) , CBU90 ( 22 ) , CBU91 ( 22 ) , chloroquine ( 6 ) , clozapine ( 30 ) , colforsin ( 32 ) , compound_C ( 20 , 26 , 29 , 40 ) , CYCLOPAMINE ( 8 ) , dantrolene ( 57 ) , dasatinib ( 16 ) , electrical_stimulation ( 10 , 24 ) , ESI-09 ( 22 ) , exercise ( 10 , 24 , 35 , 39 ) , food deprivation ( 36 ) , glucose ( 33 , 45 ) , glucose_starvation ( 24 , 58 , 64 ) , GSK'872 ( 18 ) , H-89 ( 22 ) , H2O2 ( 31 ) , HG9-91-01 ( 32 ) , high-fat diet ( 38 ) , high_glucose ( 31 ) , insulin ( 9 , 14 , 20 , 59 ) , ischemia ( 10 , 61 , 63 ) , ischemia/reperfusion ( 56 ) , KN-93 ( 57 ) , L-mevalonate ( 14 ) , leptin ( 58 ) , leucine_deprivation ( 36 ) , lncRNAs ( 15 ) , low_glucose ( 12 , 45 ) , MDL12330A ( 22 ) , metformin ( 10 , 31 , 36 , 56 , 61 ) , muscle contraction ( 59 ) , NTCU ( 60 ) , obesity-inducing diet ( 4 ) , ODN2395 ( 24 ) , oligomycin ( 61 ) , PDTC ( 66 ) , Pentadecanoic acid ( 20 ) , PF 573228 ( 8 ) , PF739 ( 24 ) , phenformin ( 25 ) , pilocarpine ( 23 ) , pomegranate_wine ( 60 ) , pravastatin ( 29 ) , pterosin_B ( 32 ) , pyridoxine ( 26 ) , Q-VD-OPh ( 18 ) , rapamycin ( 36 ) , serum_starvation ( 9 ) , simvastatin ( 14 ) , siRNA ( 7 ) , stiff ECM substrate ( 8 ) , STO-609 ( 22 , 57 , 59 ) , SU6656 ( 49 ) , sucrose ( 66 ) , telmisartan ( 31 ) , tempol ( 29 ) , TNF ( 18 ) , Trametinib ( 15 ) , U73122 ( 22 ) , VAT-R ( 17 ) , virus infection ( 13 ) , Z-VAD-FMK ( 18 )

Downstream Regulation
Effects of modification on AMPKA2:
enzymatic activity, induced ( 18 , 21 , 24 , 29 , 33 , 58 , 64 , 66 ) , intracellular localization ( 58 ) , phosphorylation ( 18 , 21 ) , protein degradation ( 19 )
Effects of modification on biological processes:
autophagy, induced ( 10 , 16 , 18 ) , autophagy, inhibited ( 8 , 11 ) , carcinogenesis, induced ( 8 ) , carcinogenesis, inhibited ( 15 , 16 ) , cell differentiation, inhibited ( 33 ) , cell growth, inhibited ( 15 ) , cell motility, inhibited ( 15 ) , cytoskeletal reorganization ( 19 ) , signaling pathway regulation ( 4 , 7 , 20 ) , transcription, altered ( 17 , 58 ) , transcription, induced ( 29 ) , transcription, inhibited ( 4 , 26 )

Disease / Diagnostics Relevance
Relevant diseases:
Alzheimer's disease ( 11 )

References 

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2

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3

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4

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6

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7

Li J, Wang Y, Chen D, Liu-Bryan R (2021) Oral administration of berberine limits post-traumatic osteoarthritis development and associated pain via AMP-activated protein kinase (AMPK) in mice. Osteoarthritis Cartilage
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8

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9

Zhou B, et al. (2021) Serum- and glucocorticoid-induced kinase drives hepatic insulin resistance by directly inhibiting AMP-activated protein kinase. Cell Rep 37, 109785
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10

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11

Hou Y, et al. (2021) NAD supplementation reduces neuroinflammation and cell senescence in a transgenic mouse model of Alzheimer's disease via cGAS-STING. Proc Natl Acad Sci U S A 118
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12

Tang X, et al. (2021) Combined intermittent fasting and ERK inhibition enhance the anti-tumor effects of chemotherapy via the GSK3β-SIRT7 axis. Nat Commun 12, 5058
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13

Li Z, et al. (2021) Porcine Haemagglutinating Encephalomyelitis Virus Triggers Neural Autophagy Independent of ULK1. J Virol, JVI0085121
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14

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15

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16

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17

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18

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19

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20

Fu WC, et al. (2021) Pentadecanoic acid promotes basal and insulin-stimulated glucose uptake in C2C12 myotubes. Food Nutr Res 65
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21

Song TJ, et al. (2021) Antidiabetic effects of betulinic acid mediated by the activation of the AMP-activated protein kinase pathway. PLoS One 16, e0249109
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22

Park SJ, et al. (2021) Potent PDE4 inhibitor activates AMPK and Sirt1 to induce mitochondrial biogenesis. PLoS One 16, e0253269
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23

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24

Liu Y, et al. (2020) TLR9 and beclin¿¿1 crosstalk regulates muscle AMPK activation in exercise. Nature
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25

Di Magno L, et al. (2020) Phenformin Inhibits Hedgehog-Dependent Tumor Growth through a Complex I-Independent Redox/Corepressor Module. Cell Rep
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26

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27

Zuo A, et al. (2020) CTRP9 knockout exaggerates lipotoxicity in cardiac myocytes and high-fat diet-induced cardiac hypertrophy through inhibiting the LKB1/AMPK pathway. J Cell Mol Med
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28

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29

Ma H, et al. (2017) Pravastatin activates activator protein 2 alpha to augment the angiotensin II-induced abdominal aortic aneurysms. Oncotarget 8, 14294-14305
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30

Choi Y, et al. (2017) Clozapine Improves Memory Impairment and Reduces Aβ Level in the Tg-APPswe/PS1dE9 Mouse Model of Alzheimer's Disease. Mol Neurobiol 54, 450-460
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31

Shang F, et al. (2016) Cardiovascular Protective Effect of Metformin and Telmisartan: Reduction of PARP1 Activity via the AMPK-PARP1 Cascade. PLoS One 11, e0151845
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32

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33

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34

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35

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36

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37

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38

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39

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40

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41

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42

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43

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44

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45

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46

Guo A (2011) CST Curation Set: 12478; Year: 2011; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
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47

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48

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49

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50

Zhou J (2009) CST Curation Set: 7426; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
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51

Zhou J (2009) CST Curation Set: 7413; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
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52

Zhou J (2009) CST Curation Set: 7425; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
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53

Zhou J (2009) CST Curation Set: 7418; Year: 2009; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
Curated Info

54

Zhou J (2009) CST Curation Set: 7414; Year: 2009; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
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55

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56

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57

Jensen TE, et al. (2007) Caffeine-induced Ca(2+) release increases AMPK-dependent glucose uptake in rodent soleus muscle. Am J Physiol Endocrinol Metab 293, E286-92
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58

Suzuki A, et al. (2007) Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha2 form of AMP-activated protein kinase. Mol Cell Biol 27, 4317-27
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59

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60

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61

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62

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63

Sakamoto K, et al. (2006) Deficiency of LKB1 in heart prevents ischemia-mediated activation of AMPKalpha2 but not AMPKalpha1. Am J Physiol Endocrinol Metab 290, E780-8
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64

Jones RG, et al. (2005) AMP-activated protein kinase induces a p53-dependent metabolic checkpoint. Mol Cell 18, 283-93
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65

Dobrzyn P, et al. (2004) Stearoyl-CoA desaturase 1 deficiency increases fatty acid oxidation by activating AMP-activated protein kinase in liver. Proc Natl Acad Sci U S A 101, 6409-14
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66

Daniel PV, et al. NF-κB p65 regulates hepatic lipogenesis by promoting nuclear entry of ChREBP in response to a high carbohydrate diet. J Biol Chem 296, 100714
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